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英文名稱	Annexin V
中文名稱	重組膜粘連蛋白5抗體
別名	Anchorin CI; Annexin 5; Annexin A5; Annexin V; Annexin5; AnnexinA5; AnnexinV; ANX 5; ANX A5; ANX5; ANXA5; Calphobindin I; CBP I; CBP-I; Endonexin II; ENX 2; ENX2; Lipocortin V; PAP I; Placental anticoagulant protein I; PAP-I; Placental anticoagulant protein 4; PP 4; PP4; Thromboplastin inhibitor; VAC alpha; Vascular anticoagulant alpha; Vascular anticoagulant-alpha; ANXA5_HUMAN; Anchorin CII; Annexin-5; VAC-alpha.
研究領(lǐng)域	細胞凋亡
抗體來源	Rabbit
克隆類型	Polyclonal
交叉反應(yīng)	Human, Mouse, Rat,
產(chǎn)品應(yīng)用	WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg /test IF=1:100-500 （石蠟切片需做抗原修復(fù)） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分子量	36kDa
細胞定位	細胞膜
性狀	Liquid
濃度	1mg/ml
免疫原	recombind human Annexin V:
亞型	IgG
純化方法	affinity purified by Protein A
儲存液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed	PubMed
產(chǎn)品介紹	The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. The gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa. [provided by RefSeq, Jul 2008]. Function: Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. Subunit: Interacts with PDCD6. Post-translational modifications: S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. DISEASE: Defects in ANXA5 are associated with susceptibility to pregnancy loss, recurrent, type 3 (RPRGL3) [MIM:614391]. A common complication of pregnancy, resulting in spontaneous abortion before the fetus has reached viability. The term includes all miscarriages from the time of conception until 24 weeks of gestation. Recurrent pregnancy loss is defined as 3 or more consecutive spontaneous abortions. Similarity: Belongs to the annexin family. Contains 4 annexin repeats. SWISS: P08758 Gene ID: 308 Database links: Entrez Gene: 308 Human Entrez Gene: 11747 Mouse Entrez Gene: 25673 Rat Omim: 131230 Human SwissProt: P08758 Human SwissProt: P48036 Mouse SwissProt: P14668 Rat Unigene: 480653 Human Unigene: 1620 Mouse Unigene: 3318 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. Annexin-V是一種分子量為35～36KD的Ca2+依賴性磷脂結(jié)合蛋白，能與PS高親和力特異性結(jié)合。將Annexin-V進行熒光素（FITC、PE）或biotin標記，以標記了的Annexin-V作為熒光探針，利用流式細胞儀或熒光顯微鏡可檢測細胞凋亡的發(fā)生。其意義：檢測早期凋亡的細胞，同時可區(qū)分活細胞和壞死細胞磷脂酰絲氨酸（Phosphatidylserine，PS）正常位于細胞膜的內(nèi)側(cè)，但在細胞凋亡的早期，PS可從細胞膜的內(nèi)側(cè)翻轉(zhuǎn)到細胞膜的表面，暴露在細胞外環(huán)境中。碘化丙啶（propidine iodide，PI）是一種核酸染料，它不能透過完整的細胞膜，但在凋亡中晚期的細胞和死細胞，PI能夠透過細胞膜而使細胞核紅染。因此將Annexin-V與PI匹配使用，就可以將凋亡早晚期的細胞以及死細胞區(qū)分開來。
產(chǎn)品圖片	Sample: Lung (Mouse) Lysate at 40 ug Primary: Anti- Annexin V (bs-0450R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36 kD Observed band size: 36 kD Sample: NIH/3T3(Mouse) Cell Lysate at 30 ug Lung (Mouse) Lysate at 40 ug Primary: Anti-Annexin V (bs-0450R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36 kD Observed band size: 35 kD Sample: PANC-1(Human) Cell Lysate at 30 ug Primary: Anti-Annexin V (bs-0450R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36 kD Observed band size: 35 kD Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37← for 20 min; Incubation: Anti-Annexin V Polyclonal Antibody, Unconjugated(bs-0450R) 1:500, overnight at 4⒉C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37← for 20 min; Incubation: Anti-Annexin V Polyclonal Antibody, Unconjugated(bs-0450R) 1:500, overnight at 4⒉C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: human Neurological glioblastoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37← for 20 min; Incubation: Anti-Annexin V Polyclonal Antibody, Unconjugated(bs-0450R) 1:500, overnight at 4⒉C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37← for 20 min; Incubation: Anti-Annexin V Polyclonal Antibody, Unconjugated(bs-0450R) 1:500, overnight at 4⒉C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37← for 20 min; Incubation: Anti-Annexin V Polyclonal Antibody, Unconjugated(bs-0450R) 1:500, overnight at 4⒉C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Blank control: RSC96 (blue). Primary Antibody:Rabbit Anti-Annexin V antibody(bs-0450R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice. Antibody (bs-0450R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody of bs-0450R at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.