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中文名稱

雌激素受體α抗體

別名

Estradiol receptor; Estrogen receptor alpha; Estradiol Receptor-alpha; Estrogen Receptor 1; Atherosclerosis, susceptibility to, included; DKFZp686N23123; ER Alpha; ER; ER-alpha; ERalpha; ER[a]; Era; ESR; ESR1; ESR1_HUMAN; ESR2; ESRA; Estr; Estrogen receptor 1 (alpha); Estrogen resistance, included; HDL cholesterol, augmented response of, to hormone replacement, included; Myocardial infarction, susceptibility to, included; NR3A1; Nuclear receptor subfamily 3 group A member 1; OTTHUMP00000017718; OTTHUMP00000017719; RNESTROR.

研究領(lǐng)域	腫瘤免疫學(xué) 生長因子和激素
抗體來源	Rabbit
克隆類型	Polyclonal
交叉反應(yīng)	Human, Rat, (predicted: Mouse, Dog, Cow, Rabbit, )
產(chǎn)品應(yīng)用	WB=1:500-2000 ELISA=1:500-1000 ICC=1:100 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分子量	67kDa
細(xì)胞定位	細(xì)胞核
性狀	Liquid
濃度	1mg/ml
免疫原	KLH conjugated synthetic peptide derived from the middle of human Estrogen Receptor alpha:331-360/595
亞型	IgG
純化方法	affinity purified by Protein A
儲存液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed	PubMed
產(chǎn)品介紹	Estrogen and progesterone receptor are members of a family of transcription factors that are regulated by the binding of their cognate ligands. The interaction of hormone-bound estrogen receptors with estrogen responsive elements(EREs) alters transcription of ERE-containing genes. The carboxy terminal region of the estrgen receptor contains the ligand binding domain, the amino terminus serves as the transactivation domain, and the DNA binding domain is centrally located. Two forms of estrogen receptor have been identified, ER Alpha and ER Beta. ER Alpha and ER Beta have been shown to be differentially activated by various ligands. The biological response to progesterone is mediated by two distinct forms of the human progesterone receptor (hPR-A and hPR-B), which arise from alternative splicing. In most cells, hPR-B functions as a transcriptional activator of progesterone-responsive gene, whereas hPR-A function as a transcriptional inhibitor of all steroid hormone receptors. Function: Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Subunit: Interacts with SLC30A9 (By similarity). Binds DNA as a homodimer. Can form a heterodimer with ESR2. Interacts with NCOA3, NCOA5 and NCOA6 coactivators, leading to a strong increase of transcription of target genes. Interacts with NCOA7 in a ligand-inducible manner. Interacts with PHB2, PELP1 and UBE1C. Interacts with AKAP13. Interacts with CUEDC2. Interacts with KDM5A. Interacts with SMARD1. Interacts with HEXIM1 and MAP1S. Interacts with PBXIP1. Interaction with MUC1 is stimulated by 7 beta-estradiol (E2) and enhances ERS1-mediated transcription. Interacts with DNTTIP2, FAM120B and UIMC1. Interacts with isoform 4 of TXNRD1. Interacts with MLL2. Interacts with ATAD2 and this interaction is enhanced by estradiol. Subcellular Location: Nucleus. Post-translational modifications: Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity. Glycosylated; contains N-acetylglucosamine, probably O-linked. Similarity: Belongs to the nuclear hormone receptor family. NR3 subfamily. Contains 1 nuclear receptor DNA-binding domain. SWISS: P06211 Gene ID: 2099 Database links: Entrez Gene: 407238 Cow Entrez Gene: 403640 Dog Entrez Gene: 2099 Human Entrez Gene: 13982 Mouse Entrez Gene: 24890 Rat Omim: 133430 Human SwissProt: P49884 Cow SwissProt: P03372 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 類固醇受體（Steroid Receptors）雌激素受體ER-α（Estrogen Receptor-α, ERα）是一類位于細(xì)胞表面或細(xì)胞內(nèi)的特異性蛋白質(zhì)。某些特異的雌激素、雌激素類似物、衍生物、拮抗劑或阻斷劑與它結(jié)合后可引發(fā)或阻斷受體蛋白質(zhì)的轉(zhuǎn)化作用。并將雌激素-受體復(fù)合物從細(xì)胞質(zhì)再定位進(jìn)入細(xì)胞核以啟動蛋白質(zhì)的生物合成。
產(chǎn)品圖片	Sample:MCF-7 Cell Lysate at 30ug; Primary: Anti-Estrogen Receptor alpha (bs-0122R) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000; Predicted band size: 67 kD Observed band size: 67 kD Sample: MCF-7 Cell Lysate at 30ug; DU145 Cell Lysate at 30 ug; Primary: Anti-Estrogen Receptor alpha (bs-0122R) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000; Predicted band size: 67 kD Observed band size: 67 kDSample: Lane 1: MCF-7 (Human) Cell Lysate at 30 ug Lane 2: K562 (Human) Cell Lysate at 30 ug Lane 3: Liver (Rat) Lysate at 40 ug Lane 4: Molt-4 (Human) Cell Lysate at 30 ug Lane 5: Raji (Human) Cell Lysate at 30 ug Lane 6: Hela (Human) Cell Lysate at 30 ug Lane 7: A549 (Human) Cell Lysate at 30 ug Primary: Anti- Estrogen Receptor alpha (bs-0122R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 66/46 kD Observed band size: 62/50 kD Sample: A549(Human) Cell Lysate at 30 ug Primary: Anti-Estrogen Receptor alpha (bs-0122R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 67 kD Observed band size: 67 kD Sample: MCF-7 Cell (Human) Lysate at 30 ug Primary: Anti- Estrogen Receptor alpha (bs-0122R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 67kD Observed band size: 65kD Tissue/cell:MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Estrogen Receptor alpha) polyclonal Antibody, Unconjugated (bs-0122R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.Tissue/cell:MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Estrogen Receptor alpha) polyclonal Antibody, Unconjugated (bs-0122R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.