| 中文名稱 | 神經(jīng)元抑制蛋白抗體 |
| 別 名 | RE1-Silencing Transcription Factor; NRSF; Neural-Restrictive Silencer Factor; XBR; Neuron Restrictive Silencer Factor; Repressor Binding To The X2 Box; X2 Box Repressor; REST_HUMAN |
| 研究領域 | 神經(jīng)生物學 鋅指蛋白 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human, Mouse, (predicted: Rat, Chicken, Pig, Cow, Horse, Rabbit, ) |
| 產(chǎn)品應用 | WB=1:500-2000 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 121kDa |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NRSF:531-450/1097 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | This gene encodes a transcriptional repressor that represses neuronal genes in non-neuronal tissues. It is a member of the Kruppel-type zinc finger transcription factor family. It represses transcription by binding a DNA sequence element called the neuron-restrictive silencer element. The protein is also found in undifferentiated neuronal progenitor cells and it is thought that this repressor may act as a master negative regular of neurogenesis. Alternatively spliced transcript variants have been described [provided by RefSeq, Jul 2010] Function: Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. Transcriptional repression by REST-CDYL via the recruitment of histone methyltransferase EHMT2 may be important in transformation suppression. Subunit: Interacts with SIN3A, SIN3B and RCOR1. Interacts with CDYL. Interacts with EHMT1 and EHMT2 only in the presence of CDYL. Part of a complex containing at least CDYL, REST, WIZ, SETB1, EHMT1 and EHMT2. Subcellular Location: Nucleus. Tissue Specificity: Ubiquitous. Expressed at higher levels in the tissues of the lymphocytic compartment, including spleen, thymus, peripheral blood lymphocytes and ovary. Similarity: Contains 9 C2H2-type zinc fingers. SWISS: Q13127 Gene ID: 5978 Database links: Entrez Gene: 5978 Human Entrez Gene: 19712 Mouse Entrez Gene: 83618 Rat GenBank: AAB17211 Human Omim: 600571 Human SwissProt: Q13127 Human SwissProt: Q155C6 Mouse SwissProt: Q8VIG1 Mouse SwissProt: O54963 Rat SwissProt: O54964 Rat Unigene: 307836 Human Unigene: 631513 Human Unigene: 28840 Mouse Unigene: 482359 Mouse Unigene: 10879 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產(chǎn)品圖片 |  Sample:Hela(Human) Cell Lysate at 30 ug Hela KO NRSF (Human) Cell Lysate at 30 ug Primary: Anti- NRSF (bs-2590R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 121 kD Observed band size: 121 kD  Sample: Raji Cell Lysate at 40 ugPrimary: Anti- NRSF (bs-2590R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 121 kD Observed band size: 121 kD  Sample:Raji(Human) Cell Lysate at 30 ug Primary: Anti-NRSF (bs-2590R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 121 kD Observed band size: 121 kD Sample:Raji(Human) Cell Lysate at 30 ug Primary: Anti-NRSF (bs-2590R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 121 kD Observed band size: 121 kD  NRSF was immunoprecipitated from human hela cells lysate with bs-2590R at 1/150 dilution. Western blot was performed from the immunoprecipitate using protein A/G beads. HRP Conjugated Mouse anti-Rabbit IgG (Light Chain specific) was used as secondary antibody at 1:5000 dilution.Lane 1: human hela cells lysate 10 µg (Input). Lane 2: bs-1399R IP in human hela cells lysate. Lane 3: native rabbit IgG IP in human hela cells lysate (negative control). Secondary All lanes : Mouse anti-Rabbit IgG (Light Chain specific), HRP Conjugated, 1:5000 Tissue/cell: human gastric tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-NRSF/REST Polyclonal Antibody, Unconjugated(bs-2590R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
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