| 中文名稱 | 金屬離子轉運體1抗體 |
| 別 名 | DCT 1; DCT1; Divalent metal transporter 1; DMT 1; DMT1; Natural resistance associated macrophage protein 2; NRAMP 2; NRAMP2; NRAM2_HUMAN. |
| 研究領域 | 腫瘤 免疫學 神經生物學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human, Mouse, Rat, (predicted: Cow, ) |
| 產品應用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1ug/Test IF=1:100-500 （石蠟切片需做抗原修復） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 62kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human DMT1:251-350/568 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產品介紹 | DMT1 is a major transporter of ferrous iron from the intestinal lumen. DMT1 belongs to the family of NRAMP metal ion transporters which function in diverse organisms from bacteria to human. DMT1 (NRAMP2) transports metal ions at the plasma membrane of cells of both the duodenum and in peripheral tissues, and defective DMT1 causes anaemia. Function: Actin-bundling protein. May function in mitogen-activated protein kinase pathway. Subcellular Location: Cytoplasm. Tissue Specificity: Heart, brain, lung, skeletal muscle, and kidney. Post-translational modifications: Actin-bundling activity is abolished upon phosphorylation by cAMP-dependent protein kinase. Similarity: Belongs to the villin/gelsolin family. Contains 1 HP (headpiece) domain. SWISS: P49281 Gene ID: 4891 Database links: Entrez Gene: 4891 Human Entrez Gene: 18174 Mouse Omim: 600523 Human SwissProt: P49281 Human SwissProt: P49282 Mouse Unigene: 505545 Human Unigene: 234608 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產品圖片 |  Sample:Adrenal glands (Mouse) Lysate at 40 ug Primary: Anti-DMT1 (bs-3577R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 62 kD Observed band size: 64 kD  Paraformaldehyde-fixed, paraffin embedded (Rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DMT1) Polyclonal Antibody, Unconjugated (bs-3577R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Divalent metal transporter 1; DMT1) Polyclonal Antibody, Unconjugated (bs-3577R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Divalent metal transporter 1; DMT1) Polyclonal Antibody, Unconjugated (bs-3577R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining. Blank control:Molt-4.Primary Antibody (green line): Rabbit Anti-DMT1 antibody (bs-3577) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 20% PBST for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.  Blank control (Black line): Molt4 (Black).Primary Antibody (green line):Rabbit Anti-DMT1 antibody (bs-3577R) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647 Dilution: 3μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. |
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