| 中文名稱 | CD44抗體 |
| 別 名 | LHR; BA-1; CD 44; CD44; CD44 antigen; CD44 molecule; CD44_HUMAN; CDw44; Cell surface glycoprotein CD44; chondroitin sulfate proteoglycan 8; ECMR-III; Epican; Extracellular matrix receptor III; GP90 lymphocyte homing/adhesion receptor; hematopoietic cell E- and L-selectin ligand; Heparan sulfate proteoglycan; Hermes antigen; homing function and Indian blood group system; HSA; HUTCH-I; HUTCH1; Hyaluronate receptor; MDU2; MDU3; MIC4; MUTCH1; PGP-1; PGP1; Phagocytic glycoprotein 1; Phagocytic glycoprotein I; CD44 antigen isoform 1 precursor. |
| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 免疫學(xué) 干細(xì)胞 細(xì)胞凋亡 糖蛋白 t-淋巴細(xì)胞 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human, Mouse, (predicted: Rat, Dog, Cow, Horse, Rabbit, Sheep, ) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 （石蠟切片需做抗原修復(fù)） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 85kDa |
| 細(xì)胞定位 | 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CD44/HCAM/PGP1:231-330/778 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis. [provided by RefSeq, Jul 2008]. Function: Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events. Subunit: Interacts with PKN2. Interacts with HA, as well as other glycosaminoglycans, collagen, laminin, and fibronectin via its N-terminal segment. Interacts with ANK, the ERM proteins (VIL2, RDX and MSN), and NF2 via its C-terminal segment. Subcellular Location: Membrane; Single-pass type I membrane protein. Note=Colocalizes with actin in membrane protrusions at wounding edges. Tissue Specificity: Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells. Post-translational modifications: Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors. N- and O-glycosylated. O-glycosylation contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s). It is uncertain if O-glycosylation occurs on Thr-637 or Thr-638. Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672. Similarity: Contains 1 Link domain. SWISS: P16070 Gene ID: 960 Database links: Entrez Gene: 281057 Cow Entrez Gene: 960 Human Entrez Gene: 12505 Mouse Entrez Gene: 100301546 Rabbit Entrez Gene: 25406 Rat Omim: 107269 Human SwissProt: Q29423 Cow SwissProt: P16070 Human SwissProt: P15379 Mouse SwissProt: P26051 Rat Unigene: 502328 Human Unigene: 423621 Mouse Unigene: 1120 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產(chǎn)品圖片 |  Sample:Spleen(Mouse) Lysate at 40 ug Primary: Anti- CD44 (bs-4916R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 85 kD Observed band size: 120 kD  Sample:U87mg(Human) Cell Lysate at 30 ug Hela(Human) Cell Lysate at 30 ug Primary: Anti- CD44 (bs-4916R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 85 kD Observed band size: 120 kD  Sample:293T(Human) Cell Lysate at 30 ug Jurkat(Human) Cell Lysate at 30 ug Primary: Anti- CD44 (bs-4916R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 85 kD Observed band size: 115 kD  Blank control(blue):A549 (fixed with 2% paraformaldehyde for 10 min at 37℃).Primary Antibody:Rabbit Anti-CD44 antibody (bs-4916R,Green); Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.  Blank control(blue):Mouse spleen (fixed with 2% paraformaldehyde for 10 min at 37℃).Primary Antibody:Rabbit Anti-CD44 antibody (bs-4916R,Green); Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.  Blank control (blue line): U251 (fixed with 70% ethanol overnight at 4℃ and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature).Primary Antibody (green line): Rabbit Anti-CD44 antibody (bs-4916R),Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, Dilution: 1μg /test. |
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