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中文名稱

細(xì)胞色素c氧化酶IV亞型1抗體

別名

COX-IV; COX 4; COX 4I1; COX 4I2; COXIV Isoform 2; COX IV 1; COX IV-1; COX IV; COX4 2; COX4; COX4B; COX4I1; COX4I2; COX4L2; COXIV 2; COXIV; Cytochrome c oxidase polypeptide IV; Cytochrome c oxidase subunit 4 isoform 1 mitochondrial; Cytochrome C Oxidase Subunit IV; Cytochrome c oxidase subunit IV isoform 1; Cytochrome c oxidase subunit IV isoform 2 (lung); dJ857M17.2; MGC72016; COX IV-1; Cox4a Cytochrome c oxidase polypeptide IV; COX41_HUMAN; COX4I1.

研究領(lǐng)域	細(xì)胞生物免疫學(xué)
抗體來(lái)源	Rabbit
克隆類型	Polyclonal
交叉反應(yīng)	Human, Mouse, Rat, (predicted: Dog, Pig, Cow, Horse, )
產(chǎn)品應(yīng)用	WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分子量	17kDa
細(xì)胞定位	細(xì)胞漿細(xì)胞膜
性狀	Liquid
濃度	1mg/ml
免疫原	KLH conjugated synthetic peptide derived from human COX4:51-169/169
亞型	IgG
純化方法	affinity purified by Protein A
儲(chǔ) 存液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed	PubMed
產(chǎn)品介紹	Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. [provided by RefSeq, Jul 2008] Function: This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. Subcellular Location: Mitochondrion inner membrane. Post-translational modifications: Ubiquitous. Similarity: Belongs to the cytochrome c oxidase IV family. SWISS: P13073 Gene ID: 1327 Database links: Entrez Gene: 1327 Human Entrez Gene: 12857 Mouse Entrez Gene: 29445 Rat Entrez Gene: 281090 Cow Omim: 123864 Human SwissProt: P00423 Cow SwissProt: Q3SZS0 Cow SwissProt: P13073 Human SwissProt: P19783 Mouse SwissProt: P10888 Rat Unigene: 433419 Human Unigene: 386758 Mouse Unigene: 2528 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品圖片	Sample: kidney (Mouse) Lysate at 40 ug Primary: Anti-COX4 (bs-10257R) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1/5000 dilution Predicted band size: 17 kD Observed band size: 17 kD Sample: heart (Mouse) Lysate at 40 ug Primary: Anti-COX4 (Bs-10257R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 17 kD Observed band size: 17 kD Sample: kidney (Mouse) Lysate at 40 ug Primary: Anti-COX4 (bs-10257R) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1/5000 dilution Predicted band size: 17 kD Observed band size: 17 kD Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (COX4) Polyclonal Antibody, Unconjugated (bs-10257R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-COX4 Polyclonal Antibody, Unconjugated(bs-10257R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Positive control: Hela cells Concebtration: 5μg/10^6 cells Incubation conditions: Avoid light , 30 minutes on the ice.