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干擾素β抗體

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中文名稱 干擾素β抗體
別    名 Interferon beta; Fibroblast interferon; IFB; IFF; IFN beta; IFNB 1; IFNB; IFNB1; Interferon beta 1 fibroblast; Interferon beta precursor; MGC96956.  

 

研究領(lǐng)域 腫瘤  細(xì)胞生物  免疫學(xué)  干擾素  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human, 
產(chǎn)品應(yīng)用 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (石蠟切片需做抗原修復(fù))
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 20kDa
細(xì)胞定位 分泌型蛋白 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human IFN-Beta:101-187/187 
亞    型 IgG
純化方法 affinity purified by Protein A
儲(chǔ) 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
產(chǎn)品介紹 Interferon-Beta, IFN-Beta mainly by the fibroblast cell factor, belonging to a single gene encodes a protein that can interfere with the virus RNA or DNA replication and inhibit virus growth, and significantly enhance NK cell activity, through the promotion of MHC Ⅰ molecule expression and enhance the CTL of the virus-infected cells role in the identification and destruction.

Function:
Has antiviral, antibacterial and anticancer activities.

Subunit:
Monomer.

Subcellular Location:
Secreted.

Similarity:
Belongs to the alpha/beta interferon family.

SWISS:
P01574

Gene ID:
3456

Database links:

Entrez Gene: 3456 Human

Omim: 147640 Human

SwissProt: P01574 Human

Unigene: 93177 Human



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

β型干擾素(Interferon-β,IFN-β)是主要由成纖維細(xì)胞產(chǎn)生的細(xì)胞因子,屬于單一基因編碼產(chǎn)生的蛋白質(zhì),可通過干擾病毒RNA或DNA復(fù)制而抑制病毒生長(zhǎng),并可顯著增強(qiáng)NK細(xì)胞殺傷活性,通過促進(jìn)MHCⅠ類分子表達(dá)而增強(qiáng)CTL對(duì)病毒感染細(xì)胞的識(shí)別和殺傷作用.
產(chǎn)品圖片 Paraformaldehyde-fixed, paraffin embedded (human pancreatic cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IFN-Beta/Ifnb1) Polyclonal Antibody, Unconjugated (bs-0787R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IFN-Beta/Ifnb1) Polyclonal Antibody, Unconjugated (bs-0787R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Tissue/cell: human colon cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-IFN-Beta/Ifnb1 Polyclonal Antibody, Unconjugated(bs-0787R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human colon cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-IFN-Beta/Ifnb1 Polyclonal Antibody, Unconjugated(bs-0787R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (human endometrial carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IFN-Beta/Ifnb1) Polyclonal Antibody, Unconjugated (bs-0787R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

 

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