| Synonyms: | COLO-699（人肺癌腺癌細(xì)胞）COLO-699 |
| Background: | established from the pleural fluid of a 57-year-old woman with adenocarcinoma of the lung in 1986 |
| Species: | human (Homo sapiens) |
| Tissue: | lung |
| Disease: | lung adenocarcinoma |
| Gender: | N/A |
| Morphology: | adherent, epitheloid cells growing as monolayers |
| Growth Mode: | N/A |
| Doubling Time: | ca. 38 hours |
| DNA Profile: | N/A Cell Line Authentication Service |
| Culture Medium: | RPMI-1640+10%FBS We strongly suggest to purchase the complete medium from |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization assays |
| Immunology: | cytokeratin -, cytokeratin-7 -, cytokeratin-8 -, cytokeratin-17 -, cytokeratin-18 -, desmin -, endothel -, EpCAM -, GFAP -, neurofilament -, vimentin +; cells are HMB-45 -positive (this marker is highly associated with melanomas - hence the cells may be melanoma-derived) |
| Fingerprint: | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Species: | confirmed as human with IEF of AST, MDH |
| Cytogenetics: | human hypertriploid karyotype with 2% polyploidy - 70(62-72)<3n>XX, -X, +1, +3, -4, +6, +7, +7, +8, -9, -10, +12, -14, -16, -17, -17, +18, +20, der(2)t(2;10)(q27;q12), der(7)t(7;?)(p21;?)x2, i(10q), der(11)t(8;11)(q12;q14), idic(16q), der(21)t(21;?)(p12;?)x2 - a supernumerary small ring chromosome was present in 28% cells - corresponds to original karyotype with reinterpretation |
| Virus | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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