| Synonyms: | EFM-192B |
| Background: | established from the pleural effusion of the left side (14 days after EFM-192A) of a 46-year-old Caucasian woman with breast adenocarcinoma; cells were described in the literature to overexpress the erB-2 gene; sister cell lines EFM-192A and -192C show differences in molecular aspects |
| Species: | human (Homo sapiens) |
| Tissue: | breast |
| Disease: | breast carcinoma |
| Gender: | N/A |
| Morphology: | epitheloid adherent cells growing as monolayers |
| Growth Mode: | N/A |
| Doubling Time: | ca. 4-5 days |
| DNA Profile: | N/A Cell Line Authentication Service |
| Culture Medium: | PMI-1640+20%FBS We strongly suggest to purchase the complete medium from . |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization assays |
| Immunology: | cytokeratin +, cytokeratin-7 +, cytokeratin-8 -, cytokeratin-17 +, cytokeratin-18 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin - |
| Fingerprint: | same DNA profile as EFM-192A/EFM-192C using multiplex PCR of minisatellite markers |
| Species: | confirmed as human with IEF of LDH, MDH, NP |
| Cytogenetics: | human heteroploid hypotriploid karyotype with 13% polyploidy - 62(58-62)<3n>XXX/XX, -X, +2, +4, +6, -7, -8, -8, -10, -11, -12, +14, +16, -17, -17, -17, -18, -18, -19, -20, -21, -22, +8mar, add(1)(p22-31), add(2)(q?35), del(3)(p22), add(5)(p15), del(6)(q25), add(10)(p11), add(13)(q32), add(14)(p12), add(15)(p12), der(15)t(10;15)(q11;p12), der(20)t(1;20)(q24;q12/13), der(21)t(7;21)(q11;p12)x2 - most cells carry a small ring and 1-2 large submetacentric 15p+ markers - closely related to EFM-192A - apparent ch 17 nullisomy |
| Virus | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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