| Synonyms: | HH-8人胚腎細(xì)胞 |
| Background: | The cell line HH-8 was generated by transfecting a vector (pcDNA3) containing a truncated calcium channel cDNA into (HEK) 293 cells (ECACC catalogue no. 85120602). The vector is constituitively expressed in the HH-8 cells producing a truncated (at amino acid 1733) cardiac alpha 1c-a calcium channel. |
| Species: | human (Homo sapiens) |
| Tissue: | Kidney, embryo |
| Disease: | Human embryo kidney |
| Gender: | N/A |
| GMO Status: | Genetically Modified Organism Class 1 (GMO1) |
| Growth Mode: | Semi-adherent aggregates |
| Doubling Time: | N/A |
| DNA Profile: | Cell Line Authentication Service |
| Culture Medium: | DMEM+10%FBS We strongly suggest to purchase the complete medium |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Karyotype: | Not specified |
| Subculture Routine: | Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin, trypsin/EDTA, or PBS wash, 5% CO2; 37°C. Cells may take up to 7 days to attach after resuscitation and sub culture. Cells grow in large aggregates that do not grow to confluency. Cells detach easily at room temperature or during transit, therefore growing cultures may be received with cells in suspension. In this event, centrifuge contents of flask and re-seed to allow re-attachment of cells. |
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