| Synonyms: | VM-CUB-1 |
| Background: | established from the primary transitional cell carcinoma of the urinary bladder of a male patient; described in the literature to carry a p53 point mutation |
| Species: | human (Homo sapiens) |
| Tissue: | urinary bladder |
| Disease: | urinary bladder transitional cell carcinoma |
| Gender: | N/A |
| Morphology: | adherent epithelial-like cells growing as monolayers; |
| Growth Mode: | N/A |
| Doubling Time: | ca. 30-45 hours |
| DNA Profile: | N/A Cell Line Authentication Service |
| Culture Medium: | DMEM+10%FBS We strongly suggest to purchase the complete medium from |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Mycoplasma: | contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin - |
| Fingerprint: | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Species: | confirmed as human with IEF of AST, NP |
| Cytogenetics: | human hypertriploid karyotype with 4% polyploidy - 80<3n>XX/XX?Y, +1, +1, +5, +6, +7, +9, -11, +15, -18, +19, +20, +22, +22, +7mar, del(1)(q21), del(1)(q11), der(1;6)(p10;q10)x2, add(3)(p21.3)x2, i(5p), der(6)t(1;6)(q11;q22), ?inv(7)(p15q35), i(9q), del(10)(p11), del(10)(q?22?24), add(12)(p11)x2, add(15)(p11), add(21)(p11) - sideline with i(5q) |
| Virus | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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