概述
FOCUS(TM) Glycoprotein kit is based on lectin binding of specific glycoproteins with terminal α-D mannosyl and α-D glycosyl proteins. FOCUS(TM) Glycoprotein kit isolates glycoproteins from complex biological solutions using spin columns that contain lectin (Concanavalin A) bound resin with the capacity to bind and immobilize ~5 mg glycoproteins. Column bound glycoproteins are eluted with a set of three rapid elution buffers. The proprietary serial elution allows for faster elution compared to other glycoprotein purification systems.
特點(diǎn)
- Spin column protocol. Uses a high capacity lectin binding resin (10~16 mg/ml resin);
- Elution of glycoproteins within 90 minutes with a set of three rapid elution buffers.
應(yīng)用
Fractionation and enrichment of glycoproteins suitable for 2D eletrophoresis and other applications.
組份
Glyco-Loading Buffer; Glyco-Elution Buffer-I; Glyco-Elution Buffer-II; Glyco-Elution Buffer-III; Glyco-Columns
