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FITC anti-human CD197 (CCR7) Antibody

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Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
CCR7-transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser
Blue Laser (488 nm)
Additional Product Notes

View more applications data for this product in our Scientific Poster Library.

Product Citations
  1. Adams S, et al. 2012. Clin Cancer Res. 18:6748. PubMed
  2. Conforti A, et al. 2021. PLoS One. 16:e0259894. PubMed
  3. Li H, et al. 2016. J Immunol. 196: 4064 - 4074. PubMed
  4. Hearnden R, et al. 2021. STAR Protocols. 2(2):100422. PubMed
  5. Zebley CC, et al. 2021. Cell Rep. 37:109796. PubMed
  6. Vanshylla K, et al. 2022. Cell Host Microbe. 30:69. PubMed
  7. Rubio-Pérez C, et al. 2021. Nat Commun. 1503:12. PubMed
  8. Dan H, et al. 2020. Mol Oncol. 14:795. PubMed
  9. Tarke A, et al. 2021. Cell Reports Medicine. 2(7):100355. PubMed
  10. Peluso MJ, et al. 2021. Cell Rep. 36:109518. PubMed
  11. Kim D, et al. 2020. Nat Med. 26:236. PubMed
  12. Rebou?as A, et al. 2021. Microorganisms. 9:. PubMed
  13. Zebley CC, et al. 2021. Cell Rep. 37:110079. PubMed
  14. Mudd PA, et al. 2022. Cell. 185:603. PubMed
  15. Yu ED, et al. 2022. Cell Host Microbe. 30:1269. PubMed
  16. Einkauf KB, et al. 2019. J Clin Invest. 129:988. PubMed
  17. Einkauf KB, et al. 2022. Cell. 185:266. PubMed
  18. Jiang C, et al. 2020. Nature. 261:585. PubMed
  19. Lazarski CA, et al. 2020. Cytotherapy. . PubMed
  20. Sehgal R, et al. 2022. Front Immunol. 13:828949. PubMed
  21. Schouest B, et al. 2021. J Virol. 95:. PubMed
  22. Fu Y, et al. 2021. Cell Reports. 36(2):109344. PubMed
  23. Qin H, et al. 2015. Blood. 126: 629 - 639. PubMed
  24. Rousso-Noori L, et al. 2021. Nat Commun. 12:3615. PubMed
  25. Hackstein CP, et al. 2022. Nat Commun. 13:7472. PubMed
  26. Wang B, et al. 2018. Mol Ther Nucleic Acids. 0.548611111. PubMed
  27. Yu ED, et al. 2022. Cell Host Microbe. . PubMed
  28. Sasawatari S, et al. 2020. J Immunol. 1373:204. PubMed
  29. Hu W, et al. 2022. Mater Today Bio. 14:100224. PubMed
RRID
AB_10945291 (BioLegend Cat. No. 353215) AB_10916386 (BioLegend Cat. No. 353216)

Antigen Details

Structure
Chemokine receptor, G protein-coupled receptors (GPCR), seven transmembrane receptor.
Distribution

T cells, B cells, NK, dendritic cells.

Function
The chemokine receptor CCR7 plays a pivotal role in the homing of na?ve T cells and regulatory T cells to secondary lymphoid organs, and the migration of dendritic cells into afferent lymphatic vessels.
Ligand/Receptor
CCL19 and CCL21.
Cell Type
B cells, Dendritic cells, NK cells, T cells
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Yanagihara S,　et al.　1998.　J. Immunol.　161:3096.
2. Charo IF,　et al.　2006.　N. Engl. J. Med.　354:610.
3. Reif K,　et al.　2002.　Nature　416:94.
4. Nakata B,　et al.　2008.　Oncology　74:69.
5.　Brodie T.　et al. 2013.　Cytometry A.　6: 530-2.　PubMed
6. Graves A.J. et al. 2014. Cytometry A. 7: 576–9　PubMed
7. Moncunill G. et al. 2014. Cytometry A. 12: 995-8　PubMed

Gene ID
1236 View all products for this Gene ID
UniProt
View information about CD197 on UniProt.org
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