Product Details

Verified Reactivity

Human, Mouse, Rat, All species

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

KLH-conjugated His-tag peptide.

Formulation

This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.

Preparation

The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.

Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is?0.025-1 ?g per ml (1:500-1:20000 dilution). It is recommended that the reagent be titrated for optimal performance for each application.

25 ?l and 100 ?l of Direct-Blot? HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.

Application Notes

This antibody was raised against a linear 8X His tag. It reacts with proteins displaying repeats of 6 Histidines (His6) or more in either internal or C and N terminal regions. It is suitable for ICFC.

Clone J095G46 has been verified for　immunocytochemistry (ICC).

RRID

AB_2734398 (BioLegend Cat. No. 362613) AB_2734399 (BioLegend Cat. No. 362614)