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FITC anti-His-Tag Antibody

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Product Details

Verified Reactivity
Human, Mouse, Rat, All species
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
KLH-conjugated His-tag peptide.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?L per million cells in 100 ?L staining volume or 5 ?L per 100 ?L of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

This antibody was raised against a linear 8X His tag. It reacts with proteins displaying repeats of 6 Histidines (His6) or more in either internal or C and N terminal regions. It is suitable for ICFC.

Clone J095G46 has been verified for immunocytochemistry (ICC).

RRID
AB_2904384 (BioLegend Cat. No. 362618)

Antigen Details

Structure
6 repeat His amino acid
Function
Epitope tag
Biology Area
Cell Biology, Immunology
Antigen References
  1. Zhao C, et al. 2010. Anal Biochem. 399:237
  2. Salichs E, et al. 2009. PLoS Genet. 5:e1000397
  3. Terpe K. 2003. Appl. Microbiol. Biotechnol. 60:523
  4. Lindner, P, et al. 1997. Biotechniques. 22:140
  5. Burritt JB, et al. 1995. J. Biol. Chem. 270:16974.
  6. Taylor RM, et al. 2007. Methods Mol. Biol. 412:429.
Gene ID
NA
UniProt
View information about His Tag on UniProt.org
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