Product Details

Verified Reactivity

Human, Mouse, Rat, All species

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

KLH-conjugated His-tag peptide.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

This antibody was raised against a linear 8X His tag. It reacts with proteins displaying repeats of 6 Histidines (His6) or more in either internal or C and N terminal regions. It is suitable for ICFC.

Clone J095G46 has been verified for　immunocytochemistry (ICC).

Product Citations

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12. Jandke A, et al. 2020. Nat Commun. 3.075694444. PubMed
13. Long Y, et al. 2021. Biochem Biophys Res Commun. 571:152. PubMed
14. van der Schoot JMS, et al. 2019. Sci Adv. 5:eaaw1822. PubMed
15. Sokal A, et al. 2021. Immunity. S1074-7613:00396. PubMed
16. Alberts A, et al. 2020. Front Immunol. 11:596103. PubMed
17. Wang R, et al. 2021. Immunity. 54:1611. PubMed
18. Zhang M, et al. 2022. iScience. 25:104490. PubMed

RRID

AB_2563634 (BioLegend Cat. No. 362603)