Product Details

Verified Reactivity

Human, Mouse, Rat, All species

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

KLH-conjugated His-tag peptide.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

WB -　Quality tested
ICC, FC -　Verified

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1:1000-1:10000 diluted (0.05 -?0.5 ?g/mL).?For immunocytochemistry, a concentration range of?0.1 - 1.0 ?g/mL (1:500-1:5000 dilution) is recommended.?For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 ?g per million cells in 100 ?L?volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody was raised against a linear 8X His tag. It reacts with proteins displaying repeats of 6 Histidines (His6) or more in either internal or C and N terminal regions. It is suitable for ICFC.

Clone J095G46 has been verified for　immunocytochemistry (ICC).

Product Citations

1. Zhang S, et al. 2021. Front Oncol. 585457:11. PubMed
2. Sokal A, et al. 2021. Cell. 184(5):1201-1213.e14. PubMed
3. Alberts A, et al. 2020. Front Immunol. 11:596103. PubMed

RRID

AB_2721736 (BioLegend Cat. No. 362601) AB_2721737 (BioLegend Cat. No. 362602)