Product Details

Verified Reactivity

All species (6x His tag epitope)

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

KLH-conjugated His-tag peptide

Formulation

This antibody is provided in 50% glycerol in aqueous buffered solution with preservatives.

Preparation

The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light. Avoid exposure to sodium azide.

Application

ELISA - Quality tested
WB - Verified

Recommended Usage

Each lot of this antibody is quality control tested by direct ELISA assay. For direct ELISA, a concentration range of 0.01 - 1.0 ?g per ml is recommended. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 ?g per ml. Avoid using diluent solutions containing sodium azide. Sodium azide is an inhibitor of horseradish peroxidase. It is recommended that the end-user titrate each lot of reagents for optimal performance.

Application References

(PubMed link indicates BioLegend citation)

1. Burritt JB, et al. 1995. J. Biol. Chem. 270:16974.
2. Taylor RM, et al. 2007. Methods Mol. Biol. 412:429.

Product Citations

1. Hay CE, et al. 2020. J Pharmacol Exp Ther. 16:374. PubMed
2. Bojadzic D, et al. 2021. Front Pharmacol. 11:600372. PubMed
3. Bojadzic D, et al. 2021. ACS Infect Dis. 7:1519. PubMed
4. Wang HC, et al. 2020. Antimicrob Agents Chemother. 65:. PubMed
5. Zhang D, et al. 2020. Oncoimmunology. 9:1744921. PubMed
6. Chen X, et al. 2021. Nat Commun. 12:5506. PubMed
7. Fan Z, et al. 2020. EMBO Mol Med. 12:e11571. PubMed
8. Zhao Y, et al. 2019. Cancer Biol Ther. 0.925. PubMed
9. Zhang X, et al. 2021. Nat Chem. 13:626. PubMed
10. Chen X, et al. 2020. bioRxiv. . PubMed
11. Houben B, et al. 2020. EMBO J. e102864:39. PubMed
12. Harrasser M, et al. 2022. Breast Cancer Res. 24:39. PubMed
13. Hay CE, et al. 2018. PLoS One. 13:29. PubMed

RRID

AB_2734520 (BioLegend Cat. No. 652503) AB_2563555 (BioLegend Cat. No. 652504)