Product Details

Verified Reactivity

All species (6x His tag epitope)

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

KLH-conjugated His-tag peptide

Formulation

This antibody is provided in phosphate-buffered solution, pH 7.2, 0.09% NaN3.

Preparation

The antibody was purified by affinity chromatography.

Concentration

0.5 mg/ml

Storage & Handling

Upon receipt, store undiluted between 2°C and 8°C.

Application

WB - Quality tested
ICC　- Verified

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 0.5 ?g per ml (Dilution 1:1000 - 1:5000).?For immunocytochemistry, a concentration range of 0.1 - 0.5 μg/ml (Dilution 1:1000 - 1:5000) is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)

1. Burritt JB, et al. 1995. J. Biol. Chem. 270:16974.
2. Taylor RM, et al. 2007. Methods Mol. Biol. 412:429.

Product Citations

1. Mamedov T, et al. 2019. PLoS One. 14:e0213438. PubMed
2. Lam B, et al. 2021. Cell Rep. 37:109838. PubMed
3. Shigemori S, et al. 2017. J Dairy Sci. 10.3168/jds.2017-12872. PubMed
4. Mamedov T, et al. 2017. PLoS One.. 10.1371/journal.pone.0183589. PubMed
5. Namai F, et al. 2017. Curr Microbiol. 10.1007/s00284-017-1354-3. PubMed
6. Vunnam N, et al. 2020. Biochemistry. 59:3856. PubMed
7. Zoulias N, et al. 2021. Curr Biol. S0960-9822:01407. PubMed
8. Williford JM, et al. 2019. Sci Adv. 5:eaay1357. PubMed
9. Liu L, et al. 2019. J Cell Biol. 218:644. PubMed
10. Jain RW, et al. 2018. Cell Rep. 25:3342. PubMed
11. Mamedov T, et al. 2021. Front Plant Sci. 12:742875. PubMed
12. Manrique-Suárez V, et al. 2021. Proteins. 89:1508. PubMed
13. Yumoto K, et al. 2020. Antioxidants (Basel). 9:00. PubMed
14. Keshwani M, et al. 2015. Biochem J. 472: 329 - 338. PubMed

RRID

AB_11204080 (BioLegend Cat. No. 652501) AB_11204427 (BioLegend Cat. No. 652502) AB_2564271 (BioLegend Cat. No. 652505)