Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant Mouse CXCL5
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
ELISA Detection - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by ELISA assay. A concentration of 0.5 ?g/ml of the detection antibody was utilized to generate the example standard curve. It is recommended that the end-user titrate each lot of reagents for optimal performance.
- Application Notes
ELISA Detection: To measure human CXCL5, this antibody can be used as a detection antibody in sandwich ELISA format and paired with the purified J1119G12 antibody (Cat. No. 526202) as the capture antibody. Recombinant Human CXCL5 (ELISA Std.) (Cat. No. 573409) can be used as the protein standard.
Note: For measuring human CXCL5 in serum, plasma or cell culture supernatant, LEGEND MAX? Human CXCL5 ELISA Kit with Pre-coated Plates (Cat. No. 440907 & 440908) is specially developed and recommended.- RRID
- AB_2563678 (BioLegend Cat. No. 526303) AB_2563679 (BioLegend Cat. No. 526304)
Antigen Details
- Structure
- Chemokine
- Distribution
- Endothelial cells, fibroblasts, monocytes, macrophages, platelets, alveolar type II cells
- Function
- CXCL5 chemoattracts and activates neutrophils during inflammation, and it is induced by LPS and inflammatory cytokines. NH2-terminal truncation enhances, whereas citrullination decreases the chemotactic properties of CXCL5.
- Interaction
- Neutrophils
- Ligand/Receptor
- CXCR2
- Cell Type
- Endothelial cells, Fibroblasts, Monocytes, Macrophages, Platelets, Eosinophils
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Walz A, et al. 1991. J. Exp. Med. 174:1355.
2. Wuyts A, et al. 1999. J. Immunol. 163:6155.
3. Jeyaseelan S, et al. 2005. Am. J. Respir. Cell. Mol. Biol. 32:531.
4. Mei J, et al. 2010. Immunity 33:106.
5. Mortier A, et al. 2010. J. Biol. Chem. 285:29750.
6. Grommes J and Soehnlein O. 2011. Mol. Med. 17:293.
7. Liu Y, et al. 2011. J. Immunol. 186:3197.
8. Kuo PL, et al. 2011. J. Cell. Physiol. 226:1224.
9. Power CA, et al. 1994. Gene. 151:333.
10. Corbett MS, et al. 1994. Biochem. Biophys. Res. Commun. 205:612. - Gene ID
- 6374 View all products for this Gene ID
- UniProt
- View information about CXCL5 on UniProt.org
