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APC anti-human CD185 (CXCR5) Antibody

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Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human CXCR5-transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser
Red Laser (633 nm)
Product Citations
  1. Kim CJ, et al. 2018. Immunity. 49:1034. PubMed
  2. Kramer KJ, et al. 2022. Nat Commun. 13:3466. PubMed
  3. Aso H, et al. 2020. Cell Reports. 32(2):107887. PubMed
  4. Wild K, et al. 2021. Nat Commun. 12:6720. PubMed
  5. Vyas AK, et al. 2019. Hepatol Commun. 3:795. PubMed
  6. Sui Z, et al. 2017. Mol Med Rep. 15:4305. PubMed
  7. Chen Y, et al. 2020. Cell. 1496:183. PubMed
  8. Shuwa HA, et al. 2021. Med. 2(6):720-735.e4. PubMed
  9. Su C, et al. 2020. Nat Commun. 2.745833333. PubMed
  10. Hao H, et al. 2021. Arthritis Res Ther. 23:302. PubMed
RRID
AB_2561816 (BioLegend Cat. No. 356907) AB_2561817 (BioLegend Cat. No. 356908)

Antigen Details

Structure
G-protein coupled receptor, 7 transmembrane regions, 42 kD
Distribution

Mature B cells, Follicular helper T cells, and Burkitt's lymphoma cells.

Function
Mediates cell migration to the B cell follicles in the secondary lymphoid organs
Ligand/Receptor
CXCL13
Cell Type
B cells, Tfh
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Ma CS, et al. 2012. J. Exp. Med. 209:1241.
2. León B, et al. 2012. Nat. Immunol. 13:681.
3. Crotty S. 2011. Annu. Rev. Immunol. 29:621.
4. Kerfoot SM, et al. 2011. Immunity 34:947.
5. Sáez de Guinoa J, et al. 2011. Blood 118:1560.

Gene ID
643 View all products for this Gene ID
UniProt
View information about CD185 on UniProt.org
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