Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

mCXCR5-transfected cells

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

Clone L138D7 staining works optimally at room temperature or 4°C. Unlike other chemokine receptor antibodies, avoid using L138D7 at 37°C.

Product Citations

1. Silva M, et al. 2021. Sci Immunol. 6:eabf1152. PubMed
2. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
3. Khiew SH, et al. 2020. J Clin Invest. 130:3453. PubMed
4. Rios D, et al. 2015. Mucosal Immunol. 101038/mi. PubMed
5. Kurniawan H, et al. 2020. Cell Metabolism. 31(5):920-936. PubMed
6. Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed
7. Martínez‐López M et al. 2019. Immunity. 50(2):446-461 . PubMed
8. Xing Y, et al. 2022. Cancer Lett. 529:100. PubMed
9. Frost JN, et al. 2021. Med (N Y). 2:164. PubMed
10. Tong D, et al. 2020. Protein Cell. 0.533333333. PubMed
11. Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed

RRID

AB_2561967 (BioLegend Cat. No. 145503) AB_2561968 (BioLegend Cat. No. 145504)