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PerCP/Cyanine5.5 anti-human CD194 (CCR4) Antibody

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Product Details

Verified Reactivity
Human
Reported Reactivity
Cynomolgus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human CCR4 transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Product Citations
  1. Bacher P et al. 2019. Cell. 176(6):1340-1355 . PubMed
  2. Hinks TSC, et al. 2019. Cell Rep. 28:3249. PubMed
  3. Lintermans L, et al. 2017. Arthritis Res Ther. 10.1186/s13075-017-1343-8. PubMed
  4. Luo Y, et al. 2021. Nat Commun. 12:3913. PubMed
  5. Scottà C, et al. 2016. Haematologica. 101: 91 - 100. PubMed
  6. Gadd V, et al. 2016. PLoS One. 11: 0157771. PubMed
  7. Vanoni G, et al. 2021. eLife. 10:00. PubMed
RRID
AB_2562390 (BioLegend Cat. No. 359405) AB_2562391 (BioLegend Cat. No. 359406)

Antigen Details

Structure
GPCR, seven transmembrane receptor
Distribution

Expressed on a subset of T and B cells, basophils, monocytes, NK cells

Function
Migration of inflammatory and regulatory cells to the target tissues
Ligand/Receptor
CCL17 and CCL22
Cell Type
B cells, Basophils, Embryonic Stem Cells, Monocytes, NK cells, T cells, Tregs
Biology Area
Immunology, Innate Immunity, Stem Cells
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Katschke KJ, et al. 2001 Arthritis Rheum. 44:1022.
2. Colantonio L, et al. 2002 Eur. J. Immunol. 32:1264.
3. Jakubzick C et al. 2004 Am. J. Pathol. 165:1211.
4. Morimoto Y, et al. 2005 J. Leukoc. Biol. 78:753.
5. Baatar J, et al. 2007 J. Immunol. 178:4891.
6. Kusumoto M, et al. 2007 J. Interferon. Cytokine Res. 27:901.

Gene ID
1233 View all products for this Gene ID
UniProt
View information about CD194 on UniProt.org
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