Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human LIF, amino acids (Ser23-Phe202) (Accession# NP_002300.1), was expressed in E. coli.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
ELISA Detection - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by ELISA assay. A concentration of 2.0 ?g/ml of the detection antibody was utilized to generate the example standard curve for human LIF. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
ELISA Detection: To measure human LIF, this antibody can be used as a detection antibody in a sandwich ELISA format and paired with the purified clone Poly5233 antibody as the capture antibody. Recombinant Human LIF (ELISA Std.) can be used as the protein standard.
Note: For testing human LIF in serum, plasma, or supernatant, BioLegend’s LEGEND MAX? Human LIF ELISA Kit is specially developed and recommended.- RRID
- AB_2566783 (BioLegend Cat. No. 530301)
Antigen Details
- Structure
- Four-helix bundle fold.
- Distribution
- Expressed in the trophectoderm of the developing embryo and activated CD4+ T cells.
- Function
- LIF is a pleiotropic cytokine involved in cellular development, inflammation, immunology, and neoplasia.
- Interaction
- Embryonic stem cells.
- Ligand/Receptor
- LIF Rα, gp130
- Cell Type
- T cells
- Biology Area
- Immunology
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Metcalf D. 1991. Int. J. Cell Cloning 2:95-108.
2. Zhang JG, et al. 1998. J. Biol. Chem. 17:10798.
3. Kamohara H, et al. 2007. Int. J. Oncol. 4:977.
4. Marshall MK, et al. 1994. Endocrinology 135:141.
5. Vogiagis D, Salamonsen LA. 1999. J. Endocrinol. 160:181.
6. Zouein FA, et al. 2013. Eur. Cytokine Netw. 24:11.
7. Mathieu ME, et al. 2012. Stem Cell Rev. 8:1.
8. Hu W, et al. 2007. Nature 450:721.
9. Trouillas M, et al. 2009. Eur. Cytokine Netw. 20:51.
10. Metcalfe SM. 2011. Genes Immun. 3:157. - Gene ID
- 3976 View all products for this Gene ID
- UniProt
- View information about LIF on UniProt.org
