Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

In-house recombinant mouse EPO.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.

Concentration

0.5 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

Application

ELISA Detection - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by ELISA. For ELISA detection applications, a concentration of 0.5 ?g/mL is recommended. To obtain a linear standard curve, serial dilutions of EPO recombinant protein ranging from 500 to 7.81 mIU/mL is recommended for each ELISA plate.

It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Detection: The biotinylated M038G10 antibody is useful as a detection antibody for a sandwich ELISA assay, when used in conjunction with purified M0312E3 (Cat. No. 662202) antibody as the capture antibody.

　

Note: For testing human EPO in cell culture supernatant, serum, plasma (heparin, EDTA and citrate), urine, and other biological fluids, BioLegend's LEGEND MAX? Kits (Cat. No. 442907 & 442908) are specially developed and recommended.

RRID

AB_2650709 (BioLegend Cat. No. 502704)