Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Human MMP-9, amino acids (Ala20-Asp707) (Accession# NP_004985.2), is expressed with a C-terminal 8His tag and a linker sequence in the 293E cell line.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

ELISA Capture　- Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA Capture applications, a concentration of 4.0??g/mL is recommended. To obtain a linear standard curve, serial dilutions of MMP-9?recombinant protein ranging from 5?to 0.078 ng/mL is recommended for each ELISA plate.?It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is only tested on recombinant proteins. It may not be able to detect endogenous proteins from cell or tissue lysates by western blotting (WB), where the expression levels may be too low.

The purified M2101G05 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated M2108F07 antibody (Cat. No. 532101) as the detecting antibody.

Note:　For testing human MMP-9 in serum or plasma, BioLegend's LEGEND MAX? Kit (Cat. No. 444907) is specially developed and recommended.

RRID

AB_2566497 (BioLegend Cat. No. 681802)