Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Recombinant Human MMP-9

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

Application

ELISA Detection - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration of 0.25?g/ml is recommended. To obtain a linear standard curve, serial dilutions of human MMP-9 protein ranging from 5 to 0.078 ng/ml are recommended for each ELISA plate when paired with M2101G05 (Cat. No. 681802). It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Detection: The biotinylated M2108F07 antibody is useful as a detection antibody for a sandwich ELISA assay, when used in conjunction with purified M2101G05 (Cat. No. 681802) antibody as the capture antibody.

Note:　For testing human MMP-9 in serum or plasma, BioLegend's LEGEND MAX? Kit (Cat. No. 444907) is specially developed and recommended.

RRID

AB_2734495 (BioLegend Cat. No. 532101)