Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant Mouse IL-25
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
ELISA Detection - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by ELISA assay. For ELISA Detection applications, a concentration range of 1 - 8 ?g/mL is recommended. To obtain a linear standard curve, serial dilutions of IL-25 (IL-17E) recombinant protein ranging from 1600 to 25 pg/mL is recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
The biotinylated M3704D11 antibody is useful as the detection antibody in a sandwich ELISA assay, when used in conjunction with the pure M3706F07 antibody (Cat. No. 676304) as the capture antibody and Recombinant Recombinant Mouse IL-25 (IL-17E) (ELISA Std.) (Cat. No. 579809).
- RRID
- AB_2814449 (BioLegend Cat. No. 538104)
Antigen Details
- Structure
- Heterodimer
- Distribution
-
IL-25 is produced by mast cells, epithelial cells, alveolar macrophages, microglia, and Th2 cells
- Function
- IL-25 directly promotes Th2 cell differentiation and the expression of IL-4, IL-5, IL-13, CCL11 (eotaxin), and therefore, lung eosinophilia. IL-25 enhances IL-9 production by differentiated Th9 cells
- Interaction
- CD4+ NKT cells, macrophages, endothelial cells, eosinophils, and Th2 and Th9 cells
- Ligand/Receptor
- IL-25 binds to IL-17RB
- Antigen References
-
- Hurst SD, et al. 2002. J. Immunol. 169:443.
- Ikeda K, et al. 2003. Blood 101:3594.
- Kang CM, et al. 2005. Am. J. Respir. Cell. Mol. Biol. 33:290.
- Kleinschek ME, et al. 2007. J. Exp. Med. 204:161.
- Terashima A, et al. 2008. J. Exp. Med. 205:2727.
- Angkasekwinai P, et al. 2010. Nat. Immunol. 11:250
- Gene ID
- 140806 View all products for this Gene ID
- UniProt
- View information about IL-25 on UniProt.org
