Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Human Ig cocktail

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

FC - Quality tested
ELISA, IHC - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 ?g per 10⁶ cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The MHE-18 antibody reacts with both soluble and membrane human immunoglobulin E (IgE). It does not react with other Ig isotypes. Some formats of the MHE-18 antibody can be used as primary or secondary reagent for immunofluorescent staining or ELISA analysis.

Application References

(PubMed link indicates BioLegend citation)

1. Clark D, et al. 2013. J Immunol Methods. 22:1759. PubMed.

Product Citations

1. Balemans D,et al. 2017. Sci Rep. . 10.1038/s41598-017-12618-7. PubMed
2. Clark D, et al. 2013. J Immunol Methods. 22:1759. PubMed
3. Maehara T, et al. 2019. Medicine (Baltimore). 98:e18300. PubMed
4. Vivanco Gonzalez N, et al. 2022. STAR Protoc. 3:101280. PubMed
5. Eccles JD, et al. 2020. Cell Rep. 30:351. PubMed
6. Cildag S, et al. 2021. Med Pharm Rep. 94:53. PubMed

RRID

AB_830847 (BioLegend Cat. No. 325502)