Product Details

Verified Reactivity

Human

Reported Reactivity

Cynomolgus

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Human Ig cocktail

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with Pacific Blue? under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

* Pacific Blue? has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue? conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses

Excitation Laser

Violet Laser (405 nm)

Application References

(PubMed link indicates BioLegend citation)

1. Lockridge JL, et al. 2013. Biol. Blood Marrow Transplant 9:1310-22. (ELISA)

Product Citations

1. Csomós K, et al. 2022. Nat Immunol. 23:1256. PubMed

RRID

AB_2561647 (BioLegend Cat. No. 316523) AB_2561648 (BioLegend Cat. No. 316524)