Product Details

Verified Reactivity

Human

Reported Reactivity

African Green, Baboon, Cynomolgus, Rhesus

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Human Ig cocktail

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser

Blue Laser (488 nm)

Application Notes

MHM-88 antibody reacts with both soluble and membrane human immunoglobulin M (IgM). It does not react with other Ig isotypes. Additional reported applications (for the relevant formats) include: use as a primary or secondary reagent for ELISA analysis.

Due to the presence of excess soluble IgM in whole blood, which competes for antibody binding, staining for IgM on cells in whole blood is not recommended.

Application References

(PubMed link indicates BioLegend citation)

1. Perez-Shiyama C, et al. 2014. J Immunol. 192:5192. PubMed

Product Citations

1. Sajadi MM, et al. 2018. Cell. 173:1783. PubMed
2. Watanabe A, et al. 2019. Cell. 177:1124. PubMed
3. Ahmed R et al. 2019. Cell. 177(6):1583-1599 . PubMed
4. Muir L, et al. 2018. Wellcome Open Res. 2:97. PubMed
5. Fenwick C, et al. 2022. Nat Microbiol. 7:1376. PubMed
6. Lindner JM, et al. 2019. Immunity. 50:668. PubMed
7. Zost SJ, et al. 2020. Nat Med. 26:1422. PubMed
8. Frensch M, et al. 2021. Cell Mol Life Sci. 78:8165. PubMed
9. Zost SJ, et al. 2020. bioRxiv. . PubMed
10. Bonifacius A, et al. 2021. Immunity. 54(2):340-354.e6. PubMed
11. Ros F, et al. 2020. MAbs. 12:1846900. PubMed
12. Jürchott K, et al. 2016. PLoS One. 11: 0150812. PubMed
13. Lisboa F, et al. 2011. J Biol Chem. 286:7577. PubMed
14. Benhammou JN, et al. 2020. Dig Dis Sci. 66:2394. PubMed
15. Nicolò A, et al. 2022. Front Immunol. 13:1016263. PubMed
16. Rossignol ED, et al. 2021. Cell Reports. 35(8):109167. PubMed
17. Cizmeci D, et al. 2021. eLife. 10:00. PubMed
18. Fenwick C, et al. 2021. Cell Rep. 37:109814. PubMed
19. Gorman JA, et al. 2019. Front Immunol. 10:44. PubMed
20. Ju L,et al. 2017. Sci Rep.. 10.1038/s41598-017-13793-3. PubMed
21. Sanchez HN, et al. 2020. Nat Commun. 0.5. PubMed
22. Gussarow D, et al. 2021. Front Med (Lausanne). 8:770381. PubMed
23. Song S, et al. 2021. Front Immunol. 12:705140. PubMed

RRID

AB_493009 (BioLegend Cat. No. 314506)