Product Details

Verified Reactivity

Human

Reported Reactivity

African Green, Baboon, Cynomolgus, Rhesus

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Human Ig cocktail

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per 10⁶ cells in 100 ?l. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser

Blue Laser (488 nm)

Application Notes

MHM-88 antibody reacts with both soluble and membrane human immunoglobulin M (IgM). It does not react with other Ig isotypes. Additional reported applications (for the relevant formats) include: use as a primary or secondary reagent for ELISA analysis.

Due to the presence of excess soluble IgM in whole blood, which competes for antibody binding, staining for IgM on cells in whole blood is not recommended.

Additional Product Notes

BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.

Application References

(PubMed link indicates BioLegend citation)

1. Perez-Shiyama C, et al. 2014. J Immunol. 192:5192. PubMed

Product Citations

1. Carre C, et al. 2021. iScience. 24:102970. PubMed
2. Lagou V et al. 2018. Cell reports. 25(3):798-810 . PubMed
3. Sangesland M, et al. 2020. Immunity. 51(4):735-749. PubMed
4. Català-Moll F, et al. 2021. Nucleic Acids Res. 49:5057. PubMed
5. Yang Z, et al. 2018. Front Immunol. 9:2613. PubMed
6. van der Burg M, et al. 2019. Front Immunol. 10:246. PubMed
7. Tu HA, et al. 2020. Cell Rep Med. 1:100155. PubMed
8. Kohn L, et al. 2014. J Immunol. 192:5050. PubMed
9. Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed
10. Aggarwal C, et al. 2021. J Virol. 95:e0061021. PubMed
11. Flint S, et al. 2016. Haematologica. 101: 698 - 706. PubMed
12. van der Velden V, et al. 2017. J Immunol Methods. 10.1016/j.jim.2017.03.011. PubMed
13. Csomós K, et al. 2022. Nat Immunol. 23:1256. PubMed
14. Rother M, et al. 2016. J Immunol. 197: 441 - 448. PubMed
15. Upasani V, et al. 2019. Front Immunol. 2.152777778. PubMed
16. van Dongen JJM, et al. 2019. Front Immunol. 10:1271. PubMed

RRID

AB_961367 (BioLegend Cat. No. 314511) AB_2076098 (BioLegend Cat. No. 314512)