Product Details
- Verified Reactivity
- Human
- Reported Reactivity
- African Green, Baboon, Cynomolgus, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human Ig cocktail
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
ELISA - Reported in the literature, not verified in house - Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 ?g per 106 in 100 ?l staining volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
MHM-88 antibody reacts with both soluble and membrane human immunoglobulin M (IgM). It does not react with other Ig isotypes. Additional reported applications (for the relevant formats) include: use as a primary or secondary reagent for ELISA analysis.
Due to the presence of excess soluble IgM in whole blood, which competes for antibody binding, staining for IgM on cells in whole blood is not recommended.- Application References
(PubMed link indicates BioLegend citation) -
- Perez-Shiyama C, et al. 2014. J Immunol. 192:5192. PubMed
- Product Citations
-
- RRID
- AB_493003 (BioLegend Cat. No. 314502)
Antigen Details
- Structure
- Ig family
- Distribution
-
B cells
- Function
- B cell differentiation, humoral immune response; cross-linking surface IgM induces apoptosis
- Cell Type
- B cells
- Biology Area
- Immunology
- Gene ID
- 3507 View all products for this Gene ID
- UniProt
- View information about IgM on UniProt.org
