Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Murine T cell hybridoma 2HB51.8

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Red Laser (633 nm)

Application Notes

Additional reported applications (for the relevant formats) include: Induction of proliferation of V?5.1⁺　and V?5.2⁺　T cells^{2, 3}　and　in vivo　depletion of V?5⁺　T cells⁴.

Application References

(PubMed link indicates BioLegend citation)

1. Kanagawa O,　et al.　1991.　J. Immunol.　147:1307. (FC)
2. Kanagawa O,　et al.　1992.　J. Immunol.　149:9. (Activ)
3. Woodland DL,　et al.　1993.　J. Exp. Med.　177:433. (Activ)
4. Gelber C,　et al.　1992.　Cancer Res.　52:6507. (Deplete)

Product Citations

1. Jayachandran R, et al. 2019. Immunity. 50:152. PubMed
2. Kashyap AS, et al. 2019. Cell Rep. 28:3367. PubMed
3. Liu X, et al. 2020. Nature. . PubMed
4. Jaeger N, et al. 2020. Cell Rep. 33:108331. PubMed

RRID

AB_10897800 (BioLegend Cat. No. 139505) AB_10933250 (BioLegend Cat. No. 139506)