Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Murine T cell hybridoma 2HB51.8
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Pacific Blue? under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody was purified by affinity chromatography and conjugated with Pacific Blue? under optimal conditions. The solution is free of unconjugated Pacific Blue?.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue? has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue? conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.?
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses- Excitation Laser
- Violet Laser (405 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: Induction of proliferation of V?5.1+ and V?5.2+ T cells2, 3 and in vivo depletion of V?5+ T cells4.
- Application References
(PubMed link indicates BioLegend citation) -
- Kanagawa O, et al. 1991. J. Immunol. 147:1307. (FC)
- Kanagawa O, et al. 1992. J. Immunol. 149:9. (Activ)
- Woodland DL, et al. 1993. J. Exp. Med. 177:433. (Activ)
- Gelber C, et al. 1992. Cancer Res. 52:6507. (Deplete)
- Product Citations
-
- RRID
- AB_2750134 (BioLegend Cat. No. 139515) AB_2750135 (BioLegend Cat. No. 139516)
Antigen Details
- Structure
- Member of the immunoglobulin superfamily, Vβ5.1and 5.2 are variants of the TCR β chain which associates with the TCR α chain to form the TCR heterodimer.
- Distribution
-
Expressed on a subset of TCR αβ+ T cells.
- Function
- Recognition of peptides presented by the MHC molecules, responsible for T cell mediated immune responses.
- Ligand/Receptor
- MHC-Peptide complex
- Cell Type
- T cells
- Biology Area
- Adaptive Immunity, Immunology
- Molecular Family
- TCRs
- Antigen References
-
1. Marrack P, et al. 2008. Annu. Rev. Immunol. 26:171.
2. Sim GK and Augustin AA. 1985. Cell 42:89.
3. Mami-Chouaib F, et al. 2002. Immunol. Rev. 188:114. - Gene ID
- 21577 View all products for this Gene ID
- UniProt
- View information about TCR Vb5.1, 5.2 on UniProt.org
