Product Details

Verified Reactivity

Human, Rat

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

White matter from bovine corpus callosum (from developer)

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide

Preparation

The antibody was purified by affinity chromatography.

Concentration

0.5 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

IHC-P - Quality tested
ICC, ICFC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry on testis samples, a concentration range of 1.25 - 5.0 µg/mL is suggested. For immunohistochemistry on cerebellum samples, a concentration range of 0.5 - 1.25 µg/mL is suggested. For immunocytochemistry, a concentration range of 2.5 - 5.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. 

Application Notes

Clone O4 recognizes specifically O4 antigen by immunocytochemistry in oligodendrocytes derived from cortical stem cells using different fixation/permeabilization methods such as methanol, PFA/Triton X-100, and PFA/methanol, where the last method shows the highest signal intensity.

Following immunohistochemistry in paraffin sections, clone O4 showed specific high staining on cerebellum white matter, and very low to none staining in the molecular layer at the recommended concentration.

By flow cytometry staining on differentiated cortical stem cells, clone O4 stained at high intensity complex cells (oligodendrocytes) compared with low complexity cells (undifferentiated progenitor stem cells).

RRID

AB_2927990 (BioLegend Cat. No. 609551)
AB_2927990 (BioLegend Cat. No. 609552)