Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 700 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

* Alexa Fluor? 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor? 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Excitation Laser

Red Laser (633 nm)

Application Notes

The OKT3 monoclonal antibody reacts with an epitope on the epsilon-subunit within the human CD3 complex.

Clone OKT3 can block the binding of clones SK7 and UCHT1.⁴ The OKT3 antibody is able to induce T cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections and activation of T cells. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 317304). For highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 317326) with a lower endotoxin limit than standard LEAF? purified antibodies (Endotoxin <0.01 EU/?g).

Application References

(PubMed link indicates BioLegend citation)

1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
2. Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
3. Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
4. Li B, et al. 2005. Immunology 116:487.
5. Jeong HY, et al. 2008. J. Leuckocyte Biol. 83:755. PubMed
6. Alter G, et al. 2008. J. Virol. 82:9668. PubMed
7. Manevich-Mendelson E, et al. 2009. Blood 114:2344. PubMed
8. Pinto JP, et al. 2010. Immunology. 130:217. PubMed
9. Biggs MJ, et al. 2011. J. R. Soc. Interface. 8:1462. PubMed

Product Citations

1. Tarke A, et al. 2021. Cell Reports Medicine. 2(2):100204. PubMed
2. Apte SH, et al. 2020. Clin Transl Immunology. 9:e1209. PubMed
3. Carbonneau S, et al. 2020. Cell Chemical Biology. 28(6):802-812.e6. PubMed
4. Gro? R, et al. 2022. EBioMedicine. 75:103761. PubMed
5. Li N, et al. 2021. STAR Protoc. 2:100942. PubMed
6. Laing AG, et al. 2020. Nat Med. 26:1623. PubMed
7. Grifoni A, et al. 2020. J Virol. 94:00:00. PubMed
8. Tian Y, et al. 2019. Cell Rep. 29:4482. PubMed
9. Wilson AS, et al. 2022. Nat Commun. 13:528. PubMed
10. Walk J, et al. 2019. Nat Commun. 10:874. PubMed
11. Chakraborty S, et al. 2022. Sci Transl Med. . PubMed
12. Tarke A, et al. 2021. Cell Reports Medicine. 2(7):100355. PubMed
13. Grifoni A, et al. 2020. Cell. 181(7):1489-1501.e15.. PubMed
14. Peluso MJ, et al. 2021. Cell Rep. 36:109518. PubMed
15. Pathania AS, et al. 2022. Mol Ther Oncolytics. 25:308. PubMed
16. Voic H, et al. 2020. J Virol. 94: . PubMed
17. Dettling S, et al. 2018. Clin Cancer Res. 24(12). PubMed
18. Reyes M, et al. 2021. Sci Transl Med. 13:. PubMed

RRID

AB_2563407 (BioLegend Cat. No. 317339) AB_2563408 (BioLegend Cat. No. 317340)