Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with APC/Cyanine7 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser

Red Laser (633 nm)

Application Notes

The OKT3 monoclonal antibody reacts with an epitope on the epsilon-subunit within the human CD3 complex.

Clone OKT3 can block the binding of clones SK7 and UCHT1.⁴ The OKT3 antibody is able to induce T cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections and activation of T cells. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 317304). For highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 317326) with a lower endotoxin limit than standard LEAF? purified antibodies (Endotoxin <0.01 EU/?g).

Additional Product Notes

BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.

Application References

(PubMed link indicates BioLegend citation)

1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
2. Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
3. Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
4. Li B, et al. 2005. Immunology 116:487.
5. Jeong HY, et al. 2008. J. Leuckocyte Biol. 83:755. PubMed
6. Alter G, et al. 2008. J. Virol. 82:9668. PubMed
7. Manevich-Mendelson E, et al. 2009. Blood 114:2344. PubMed
8. Pinto JP, et al. 2010. Immunology. 130:217. PubMed
9. Biggs MJ, et al. 2011. J. R. Soc. Interface. 8:1462. PubMed

Product Citations

1. Cirovic B, et al. 2020. Cell Host & Microbe. 28(2):322-334. PubMed
2. Cao B, et al. 2022. Nat Commun. 13:6203. PubMed
3. Wijesinghe A, et al. 2020. J Biomed Sci. 27:50:00. PubMed
4. Chow MT et al. 2019. Immunity. 50(6):1498-1512 . PubMed
5. Abdul-Jawad S, et al. 2021. Cancer Cell. 39(2):257-275.e6. PubMed
6. Laing AG, et al. 2020. Nat Med. 26:1623. PubMed
7. Dannenfelser R, et al. 2020. Cell Systems. 11(3):215-228.e5. PubMed
8. Wang LT, et al. 2020. Immunity. 53:733. PubMed
9. Jackson-Jones LH, et al. 2020. Immunity. 52:700. PubMed
10. Ye C, et al. 2017. J Virol. 91:e01389-23. PubMed
11. Tucci G, et al. 2022. Front Immunol. 13:932240. PubMed
12. Luo Y, et al. 2021. Nat Commun. 12:3913. PubMed
13. Zhong G, et al. 2022. Oncoimmunology. 11:2114740. PubMed
14. Dos Santos JC, et al. 2019. Cell Rep. 28:2659. PubMed
15. Zhang X, et al. 2021. Front Immunol. 12:602492. PubMed
16. Shen Z, et al. 2022. Immun Ageing. 19:55. PubMed
17. Rabold K, et al. 2022. Nat Commun. 13:6149. PubMed
18. Loo Yau H, et al. 2021. STAR Protocols. 2(2):100549. PubMed

RRID

AB_2563409 (BioLegend Cat. No. 317341) AB_2563410 (BioLegend Cat. No. 317342)