Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Preparation

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510? under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Brilliant Violet 510? excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510? is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Excitation Laser

Violet Laser (405 nm)

Application Notes

The OKT3 monoclonal antibody reacts with an epitope on the epsilon-subunit within the human CD3 complex.

Clone OKT3 can block the binding of clones SK7 and UCHT1.⁴ The OKT3 antibody is able to induce T cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections and activation of T cells. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 317304). For highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 317326) with a lower endotoxin limit than standard LEAF? purified antibodies (Endotoxin <0.01 EU/?g).

Application References

(PubMed link indicates BioLegend citation)

1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
2. Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
3. Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
4. Li B, et al. 2005. Immunology 116:487.
5. Jeong HY, et al. 2008. J. Leuckocyte Biol. 83:755. PubMed
6. Alter G, et al. 2008. J. Virol. 82:9668. PubMed
7. Manevich-Mendelson E, et al. 2009. Blood 114:2344. PubMed
8. Pinto JP, et al. 2010. Immunology. 130:217. PubMed
9. Biggs MJ, et al. 2011. J. R. Soc. Interface. 8:1462. PubMed

Product Citations

1. Wheatley A, et al. 2016. Sci Rep. 6: 26478. PubMed
2. Marco Barros R, et al. 2016. Cell. 167: 203-218. PubMed
3. Leeansyah E, et al. 2015. PLoS Pathog. 11: 1005072. PubMed
4. Ishizaka A, et al. 2016. J Virol. 90: 5665 - 5676. PubMed
5. Andrews SF et al. 2019. Immunity. 51(2):398-410 . PubMed
6. Lamichhane R, et al. 2020. Eur J Immunol. 50:178. PubMed
7. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
8. AC Belkina, JE Snyder-Cappione 2017. Cytometry A. 91:175-179. PubMed
9. Chiu Y, et al. 2016. Sci Rep. 6:19227. PubMed
10. Abreu C, et al. 2014. PLoS One. 9:97257. PubMed
11. Kimura I, et al. 2022. Cell Rep. 38:110218. PubMed
12. Piepenbrink MS, et al. 2021. Cell Reports Medicine. 2(3):100218. PubMed
13. Sutton HJ, et al. 2021. Cell Reports. 34(6):108684. PubMed
14. Zhou J, et al. 2015. J Immunol. 194:4688. PubMed
15. Petrelli A, et al. 2018. J Clin Invest. 128:4669. PubMed
16. Antonucci L, et al. 2020. Journal of Immunology Research. 3257.929861. PubMed
17. Juno JA, et al. 2020. Nat Med. 26:1428. PubMed
18. Kreutmair S, et al. 2021. Immunity. . PubMed
19. Herter JM, et al. 2022. Strahlenther Onkol. Online ahead of print. PubMed
20. Tan J, et al. 2021. Sci Transl Med. 13: . PubMed
21. Nguyen THO, et al. 2021. Immunity. 54:1066. PubMed
22. Yu C, et al. 2021. Med (N Y). 2:755. PubMed
23. Lu Q, et al. 2021. Immunity. 54(6):1304-1319.e9. PubMed
24. Ehlers L, et al. 2021. The FASEB Journal. 35(7):e21684. PubMed
25. Sordé L, et al. 2017. Immunity, Inflammation, and Disease. 10.1002/iid3.167. PubMed
26. van den Hoogen P, et al. 2020. PLoS One. 14:e0227283. PubMed
27. Port JR, et al. 2020. J Virol. 94:. PubMed
28. Einkauf KB, et al. 2019. J Clin Invest. 129:988. PubMed
29. Lozano-Rodríguez R, et al. 2022. Cell Rep. 38:110235. PubMed
30. Jiang C, et al. 2020. Nature. 261:585. PubMed
31. Einkauf KB, et al. 2022. Cell. 185:266. PubMed
32. Wang L, et al. 2021. Science. 373:. PubMed
33. Motozono C, et al. 2021. Cell Host Microbe. . PubMed
34. Gross C, et al. 2016. Proc Natl Acad Sci U S A. 113: 2973 - 2982. PubMed
35. Dacon C, et al. 2022. Cell Host Microbe. Online ahead of print. PubMed
36. Smalley M, et al. 2020. iScience. 23:101229. PubMed
37. Hearps AC, et al. 2017. Front Immunol. 0.840972222. PubMed
38. Cho H, et al. 2021. Sci Transl Med. 13:eabj5413. PubMed
39. Cui J, et al. 2019. Neuro Oncol. 21:1436. PubMed
40. Abd Hamid M et al. 2019. Cancer Immunol Res. 7(8):1293-1306 . PubMed
41. Seydoux E, et al. 2020. Immunity. 53:98. PubMed
42. Sant S, et al. 2020. PLoS Pathog. 16:e1008714. PubMed
43. Burian A, et al. 2016. PLoS One. 11: 0163297. PubMed
44. Aguilar–Brise? o JA, et al. 2020. Nat Commun. 2.664583333. PubMed
45. Lamichhane R et al. 2019. Cell Rep. 28(12):3061-3076 . PubMed

RRID

AB_2561376 (BioLegend Cat. No. 317331) AB_2561943 (BioLegend Cat. No. 317332)