Product Details

Verified Reactivity

Human, Cynomolgus, Rhesus

Reported Reactivity

Chimpanzee

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Human peripheral T cells

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested
IHC-F - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 5.0 - 10 ?g per ml. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses

Excitation Laser

Blue Laser (488 nm)

Application Notes

The OKT4 antibody binds to the D3 domain of CD4 and does not block HIV binding.　Additional reported applications (for the relevant formats) include: immunohistochemistry of frozen sections and blocking of T cell activation. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 317453 and 317454).

In a small subset of individuals, the OKT4 clone does not bind to CD4 due to polymorphisms in CD4.⁹

Application References

(PubMed link indicates BioLegend citation)

1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
2. Reinherz EL, et al. 1979. Proc. Natl. Acad. Sci. 76:4061.
3. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
4. Cicin-Sain L, et al. 2010. J. Immunol. 184:6739. PubMed
5. Rosenzweig M, et al. 2001. J. Med. Primatol. 30:36.
6. Linder J, et al. 1987. Am. J. Pathol. 127:1.
7. Boche D, et al. 1999. J. Neurovirol. 5:232. (IHC)
8. Reinherz EL, et al. 1979. Proc. Natl. Acad. Sci. USA. 76:4061. (Immunogen)
9. Lederman S, et al. 1991. Mol Immunol. 28:1171-81.

Product Citations

1. Demirkhanyan L, et al. 2012. J Biol Chem. 287:28821. PubMed
2. Johnson RF, et al. 2021. Viruses. 13:. PubMed
3. Rademacher MJ, et al. 2021. Sci Rep. 11:8321. PubMed
4. Porsche CE, et al. 2021. JCI Insight. 6:. PubMed
5. Glaubitz J, et al. 2022. Nat Commun. 13:4502. PubMed
6. Abe S, et al. 2020. JCI Insight. 5:00. PubMed
7. Kraig E, et al. 2018. Exp Gerontol. 105:53. PubMed
8. Zhou H, et al. 2022. EMBO Rep. 23:e53475. PubMed
9. Meyer LK, et al. 2019. J Clin Invest. 130:863. PubMed
10. Torres-Ruiz J, et al. 2021. Front Immunol. 12:689966. PubMed
11. Billing F, et al. 2021. ACS Appl Mater Interfaces. 13:55534. PubMed
12. Ellis GI, et al. 2022. STAR Protoc. 3:101784. PubMed
13. Maehara T, et al. 2018. Life Sci Alliance. 1:e201800050. PubMed
14. Singh A, et al. 2020. Cell Rep. 32:108153. PubMed
15. Hu J, et al. 2022. J Immunother Cancer. 10:. PubMed
16. Sharei A, et al. 2015. PLoS One. 10:118803. PubMed

RRID

AB_571938 (BioLegend Cat. No. 317419) AB_571939 (BioLegend Cat. No. 317420)