Product Details

Verified Reactivity

Human, Cynomolgus, Rhesus

Reported Reactivity

Chimpanzee

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Human peripheral T cells

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The OKT4 antibody binds to the D3 domain of CD4 and does not block HIV binding.　Additional reported applications (for the relevant formats) include: immunohistochemistry of frozen sections and blocking of T cell activation. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 317453 and 317454).

In a small subset of individuals, the OKT4 clone does not bind to CD4 due to polymorphisms in CD4.⁹

Application References

(PubMed link indicates BioLegend citation)

1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
2. Reinherz EL, et al. 1979. Proc. Natl. Acad. Sci. 76:4061.
3. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
4. Cicin-Sain L, et al. 2010. J. Immunol. 184:6739. PubMed
5. Rosenzweig M, et al. 2001. J. Med. Primatol. 30:36.
6. Linder J, et al. 1987. Am. J. Pathol. 127:1.
7. Boche D, et al. 1999. J. Neurovirol. 5:232. (IHC)
8. Reinherz EL, et al. 1979. Proc. Natl. Acad. Sci. USA. 76:4061. (Immunogen)
9. Lederman S, et al. 1991. Mol Immunol. 28:1171-81.

Product Citations

1. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
2. Liu M, et al. 2015. J Virol. 89:784. PubMed
3. Kanakasabapathy M, et al. 2017. Lab Chip. 10.1039/c7lc00273d. PubMed
4. Hirota K et al. 2018. Immunity. 48(6):1220-1232 . PubMed

RRID

AB_571948 (BioLegend Cat. No. 317405) AB_571949 (BioLegend Cat. No. 317406)