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產(chǎn)品編號(hào)	bs-1287R
英文名稱	Rabbit　Anti-IKB alpha　antibody
中文名稱	核因子κB抑制蛋白α抗體
別　　　　名	NFKBIA; Inhibitor of KB alpha; I kappa B alpha; I(Kappa)B(alpha); IkappaBalpha; IKBA; IKBalpha; MAD 3; MAD3; Major histocompatibility complex enhancer binding protein MAD3; NF kappa B inhibitor alpha; NFKBI; Nuclear factor of kappa light chain gene enhancer in B cells; Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha; IKBA_HUMAN; IκB-α; IκBα.
	Specific References　　(76)　　　　　\|　　　　　bs-1287R has been referenced in 76 publications. [IF=12.88]　Ma, Juan, et al. "A Crucial Role of Lateral Size for Graphene Oxide in Activating Macrophages and Stimulating Pro-inflammatory Responses in Cells and Animals." ACS Nano (2015).　　WB　;　　Mouse.　　 PubMed:26389709 [IF=10.383]　Zhen Xu. et al. Green Biosynthesis of Silver Nanoparticles Using Aqueous Extracts of Ageratum Conyzoides and Their Anti-Inflammatory Effects. ACS APPL MATER INTER. 2023;XXXX(XXX):XXX-XXX　　WB　;　　Mouse,Human.　　 PubMed:36881383 [IF=6.551]　Jing Ge. et al. Comparison of nanoparticle -selenium, selenium-enriched yeast and sodium selenite on the alleviation of cadmium-induced inflammation via NF-kB/IκB pathway in heart. Sci Total Environ. 2021 Jun;773:145442　　WB　;　　Chicken.　　 PubMed:10.1016/j.scitotenv.2021.145442 [IF=6.475]　Yongli Zhang. et al. Licorice extract ameliorates hyperglycemia through reshaping gut microbiota structure and inhibiting TLR4/NF-κB signaling pathway in type 2 diabetic mice. Food Res Int. 2022 Mar;153:110945　　WB　;　　Mouse.　　 PubMed:10.1016/j.foodres.2022.110945 [IF=6.286]　Jianhao Yang. et al. Serum amyloid A regulates TLR2/4-mediated IFN-β signaling pathway against Marek's disease virus. VIRUS RES. 2023 Jan;:199044　　WB　;　　Chicken.　　 PubMed:36652973 [IF=5.714]　Yani He. et al. Glycolytic reprogramming controls periodontitis-associated macrophage pyroptosis via AMPK/SIRT1/NF-κB signaling pathway. INT IMMUNOPHARMACOL. 2023 Jun;119:110192　　WB　;　　Human.　　 PubMed:37068341 [IF=5.561]　Jing Mao. et al. Sea Cucumber Hydrolysate Alleviates Immunosuppression and Gut Microbiota Imbalance Induced by Cyclophosphamide in Balb/c Mice through the NF-κB Pathway. FOODS. 2023 Jan;12(8):1604　　WB　;　　Mouse.　　 PubMed:37107399 [IF=5.265]　Li, Ke. et al. The Prevention Effect of Lactobacillus plantarum 17–5 on Escherichia coli-Induced Mastitis in Mice. PROBIOTICS ANTIMICRO. 2023 Feb;:1-9　　WB　;　　Mouse.　　 PubMed:36790662 [IF=5.235]　Pu, Luya. et al. Laminar shear stress alleviates monocyte adhesion and atherosclerosis development via miR-29b-3p/CX3CL1 axis regulation. J CELL SCI. 2022 Jul;135(14):　　WB　;　　Human.　　 PubMed:35735031 [IF=5.195]　Xiao Wang. et al. Kallistatin attenuates inflammatory response in rheumatoid arthritis via the NF-κB signaling pathway. EUR J PHARMACOL. 2023 Jan;:175530　　WB　;　　Mouse.　　 PubMed:36690053 [IF=5.195]　Zhen Xu. et al. The aqueous extracts of Ageratum conyzoides inhibit inflammation by suppressing NLRP3 inflammasome activation. J ETHNOPHARMACOL. 2023 Jun;309:116353　　WB　;　　Mouse,Human.　　 PubMed:36907476 [IF=5.085]　Xianmei Liu. et al. Tropomodulin1 Expression Increases Upon Maturation in Dendritic Cells and Promotes Their Maturation and Immune Functions. Front Immunol. 2020; 11: 587441　　 PubMed:33552047 [IF=4.996]　Zhao, Lei. et al. Lactobacillus plantarum S9 alleviates lipid profile, insulin resistance, and inflammation in high-fat diet-induced metabolic syndrome rats. SCI REP-UK. 2022 Sep;12(1):1-10　　WB　;　　Rat.　　 PubMed:36109620 [IF=4.936]　Ma M et al. LncRNA XIST mediates bovine mammary epithelial cell inflammatory response via NF‐κB/NLRP3 inflammasome pathway.(2018) Cell Prolif.　　WB　;　　Bovine.　　 PubMed:30362186 [IF=4.932]　Fengfeng Xie. et al. Serum Metabolomics Based on GC-MS Reveals the Antipyretic Mechanism of Ellagic Acid in a Rat Model. METABOLITES. 2022 Jun;12(6):479　　WB　;　　Rat.　　 PubMed:10.3390/metabo12060479 [IF=4.932]　Qingyu Meng. et al. Morin hydrate inhibits atherosclerosis and LPS-induced endothelial cells inflammatory responses by modulating the NFκB signaling-mediated autophagy. Int Immunopharmacol. 2021 Nov;100:108096　　WB　;　　Human.　　 PubMed:34464886 [IF=4.872]　Ge Guan. et al. The promotion of tetrabromobisphenol A exposure on Ishikawa cells proliferation and pivotal role of ubiquitin-mediated IκB′ degradation. Ecotox Environ Safe. 2021 Jan;207:111254　　WB,IP　;　　Human.　　 PubMed:32890954 [IF=4.677]　Cai, Ruping. et al. MicroRNA-136-5p protects cardiomyocytes from coronary microembolization through the inhibition of pyroptosis. Apoptosis. 2022 Jan;:1-16　　WB,IHC　;　　Rat.　　 PubMed:35084609 [IF=4.571]　Jie Xu. et al. Mechanism of nonylphenol induced gastric inflammation through NF-κB/NLRP3 signaling pathway. TOXICOLOGY. 2022 Aug;:153294　　WB　;　　Rat.　　 PubMed:35998786 [IF=4.42]　Yu, Haijie, et al. "Gypenoside Protects Cardiomyocytes against Ischemia-Reperfusion Injury via the Inhibition of Mitogen-Activated Protein Kinase Mediated Nuclear Factor Kappa B Pathway In Vitro and In Vivo." Frontiers in Pharmacology 7 (2016).　　WB　;　　Rat.　　 PubMed:27313532 [IF=4.407]　Yaping Wang. et al. Blockade of GITRL/GITR signaling pathway attenuates house dust mite-induced allergic asthma in mice through inhibition of MAPKs and NF-κB signaling. Mol Immunol. 2021 Sep;137:238　　WB,IF　;　　Mouse.　　 PubMed:34293591 [IF=4.225]　Qihe Tang. et al. Bergenin Monohydrate Attenuates Inflammatory Response via MAPK and NF-κB Pathways Against Klebsiella pneumonia Infection. Front Pharmacol. 2021; 12: 651664　　WB　;　　Mouse.　　 PubMed:34017253 [IF=4.225]　Peng L et al. Madecassoside Protects Against LPS-Induced Acute Lung Injury via Inhibiting TLR4/NF-κB Activation and Blood-Air Barrier Permeability. Front Pharmacol. 2020 Jun 5;11:807.　　WB　;　　Mouse.　　 PubMed:32581788 [IF=4.211]　Liu,et al.Human umbilical cord mesenchymal stem cell conditioned medium attenuates renal fibrosis by reducing inflammation and epithelial-to-mesenchymal transition via the TLR4/NF-κB signaling pathway in vivo and in vitro.(2018) Stem Cell Research & Therapy. 9:7.　　WB　;　　Rat.　　 PubMed:29329595 [IF=4.128]　Shiyan Chen. et al. Lactobacillus johnsonii L531 Alleviates the Damage Caused by Salmonella Typhimurium via Inhibiting TLR4, NF-κB, and NLRP3 Inflammasome Signaling Pathways. Microorganisms. 2021 Sep;9(9):1983　　WB　;　　pig.　　 PubMed:34576878 [IF=4.109]　Gao Jie-Fang. et al. Myricetin treatment has ameliorative effects in DNFB-induced atopic dermatitis mice under high-fat conditions. TOXICOL SCI. 2022 Dec;:　　WB　;　　Mouse.　　 PubMed:36575998 [IF=3.889]　Ming Dong Si. et al. Swertia mussotii prevents high-fat diet-induced non-alcoholic fatty liver disease in rats by inhibiting expression the TLR4/MyD88 and the phosphorylation of NF-κB. PHARM BIOL. 2022 Oct 07　　IHC　;　　Rat.　　 PubMed:36205548 [IF=3.845]　Peng LY et al. Protective Effect of Piceatannol Against Acute Lung Injury Through Protecting the Integrity of Air-Blood Barrier and Modulating the TLR4/NF-κB Signaling Pathway Activation. Front Pharmacol. 2020 Jan 22;10:1613.　　WB　;　　Mouse.　　 PubMed:32038265 [IF=3.69]　Xiaoyan Xia. et al. Ameliorative effect of white tea from 50-year-old tree of Camellia sinensis L. (Theaceae) on kidney damage in diabetic mice via SIRT1/AMPK pathway. J Ethnopharmacol. 2021 May;272:113919　　WB　;　　Mouse.　　 PubMed:33577915 [IF=3.654]　Yang Liu. et al. Isochlorogenic Acid C Restrains Erk/JNK/NF-κB Signaling to Alleviate Inflammatory Response and Promote Cell Apoptosis. J FOOD BIOCHEM. 2023;2023:5547108　　WB　;　　Human.　　 PubMed:10.1155/2023/5547108
研究領(lǐng)域	腫瘤　　免疫學(xué)　　信號(hào)轉(zhuǎn)導(dǎo)　　轉(zhuǎn)錄調(diào)節(jié)因子　　激酶和磷酸酶
抗體來源	Rabbit
克隆類型	Polyclonal
交叉反應(yīng)	Human,Mouse,Rat
產(chǎn)品應(yīng)用	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100, IF=1:100-500, Flow-Cyt=1μg/Test, ELISA=1:5000-10000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
理論分子量	36kDa
細(xì)胞定位	細(xì)胞核　細(xì)胞漿
性　　　　狀	Liquid
濃　　　　度	1mg/ml
免　疫　原	KLH conjugated synthetic peptide derived from human NFKBIA:　1-120/314
亞　　　　型	IgG
純化方法	affinity purified by Protein A
緩　沖　液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng)	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
產(chǎn)品介紹	This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011] Function: Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription. Subunit: Interacts with RELA; the interaction requires the nuclear import signal. Interacts with NKIRAS1 and NKIRAS2. Part of a 70-90 kDa complex at least consisting of CHUK, IKBKB, NFKBIA, RELA, IKBKAP and MAP3K14. Interacts with HBV protein X. Interacts with RWDD3; the interaction enhances sumoylation. Interacts (when phosphorylated at the 2 serine residues in the destruction motif D-S-G-X(2,3,4)-S) with BTRC. Associates with the SCF(BTRC) complex, composed of SKP1, CUL1 and BTRC; the association is mediated via interaction with BTRC. Part of a SCF(BTRC)-like complex lacking CUL1, which is associated with RELA; RELA interacts directly with NFKBIA. Interacts with PRMT2. Interacts with PRKACA in platelets; this interaction is disrupted by thrombin and collagen. Interacts with HIF1AN. Subcellular Location: Cytoplasm. Nucleus. Tissue Specificity: Highly expressed in lymph node, thymus followed by liver, brain, muscle, kidney, gastrointestinal and reproductive tract. Post-translational modifications: Phosphorylated; disables inhibition of NF-kappa-B DNA-binding activity. Phosphorylation at positions 32 and 36 is prerequisite to recognition by UBE2D3 leading to polyubiquitination and subsequent degradation. Sumoylated; sumoylation requires the presence of the nuclear import signal. Monoubiquitinated at Lys-21 and/or Lys-22 by UBE2D3. Ubiquitin chain elongation is then performed by CDC34 in cooperation with the SCF(FBXW11) E3 ligase complex, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. The resulting polyubiquitination leads to protein degradation. Also ubiquitinated by SCF(BTRC) following stimulus-dependent phosphorylation at Ser-32 and Ser-36. Deubiquitinated by porcine reproductive and respiratory syndrome virus Nsp2 protein, which thereby interferes with NFKBIA degradation and impairs subsequent NF-kappa-B activation. DISEASE: Defects in NFKBIA are the cause of ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant (ADEDAID) [MIM:612132]. Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. ADEDAID is an ectodermal dysplasia associated with decreased production of pro-inflammatory cytokines and certain interferons, rendering patients susceptible to infection. Similarity: Belongs to the NF-kappa-B inhibitor family. Contains 5 ANK repeats. SWISS: Q9Z1E3 Gene ID: 4792 Database links: Entrez Gene: 4792 Human Entrez Gene: 18035 Mouse Entrez Gene: 25493 Rat SwissProt: P25963 Human SwissProt: Q9Z1E3 Mouse SwissProt: Q63746 Rat 轉(zhuǎn)錄調(diào)節(jié)因子（Transcriptin Regulators）
產(chǎn)品圖片	Sample: Thymus gland (Mouse) Lysate at 40 ug Primary: Anti-IKB alpha (bs-1287R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36 kD Observed band size: 36 kD Sample: Spleen (mouse) Lysate at 40 ug Primary: Anti- IKB alpha (bs-1287R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36 kD Observed band size: 35 kD Sample: Spleen (Mouse) Lysate at 40 ug Primary: Anti-IKB alpha (bs-1287R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36 kD Observed band size: 36 kD Sample: skin (Mouse) Lysate at 40 ug Primary: Anti-IKB alpha (Bs- 1287R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36 kD Observed band size: 35 kD Sample: Lane 1: HepG2 (Human) Cell Lysate at 30 ug Lane 2: Hela (Human) Cell Lysate at 30 ug Lane 3: Siha (Human) Cell Lysate at 30 ug Lane 4: U251 (Human) Cell Lysate at 30 ug Lane 5: Spleen (Mouse) Lysate at 40 ug Lane 6: Thymus (Mouse) Lysate at 40 ug Lane 7: Thymus (Rat) Lysate at 40 ug Lane 8: Lymph node (Mouse) Lysate at 40 ug Lane 9: Lymph node (Rat) Lysate at 40 ug Primary: Anti-IKB alpha (bs-1287R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 39 kD Observed band size: 37 kD Sample: Lymph node (Mouse) Lysate at 40 ug Thymus (Mouse) Lysate at 40 ug Primary: Anti- IKB alpha (bs-1287R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36 kD Observed band size: 36 kD Sample: Stomach(Mouse) Lysate at 40 ug Stomach(Rat) Lysate at 40 ug Primary: Anti-IKB alpha (bs-1287R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 35 kD Observed band size: 37 kD Sample: HepG2 (Human) Cell Lysate at 30 ug Primary: Anti-IKB alpha (bs-1287R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36 kD Observed band size: 36 kD Sample: Hela(Human) Cell Lysate at 30 ug Primary: Anti-IKB alpha (bs-1287R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36 kD Observed band size: 36 kD Sample: Lung (mouse) Lysate at 40 ug Primary: Anti- IKB alpha (bs-1287R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36 kD Observed band size: 36 kD Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IKB alpha) Polyclonal Antibody, Unconjugated (bs-1287R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IKB alpha) Polyclonal Antibody, Unconjugated (bs-1287R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-IKB alpha Polyclonal Antibody, Unconjugated(bs-1287R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell:Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum,C-0005) at 37°C for 20 min; Antibody incubation with (IKB alpha) polyclonal Antibody, Unconjugated (bs-1287R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei. Blank control: Hela. Primary Antibody (green line): Rabbit Anti-IKB alpha antibody (bs-1287R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. Blank control (blue line): Jurkat (blue). Primary Antibody (green line): Rabbit Anti-IKB alpha antibody (bs-1287R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC Dilution: 1μg /test. Protocol The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.