97av麻豆蜜桃一区二区,狠狠色噜噜狠狠狠狠2021

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產(chǎn)品編號(hào)	bs-10211R
英文名稱	Rabbit　Anti-FOXP3　antibody
中文名稱	叉頭蛋白P3抗體
別　　　　名	Forkhead box protein P3; forkhead box P3; Scurfin; Forkhead box protein P3, C-terminally processed; Forkhead box protein P3 41 kDa form; JM2; AIID; IPEX; PIDX; XPID; DIETER; FOXP3_HUMAN;
	Specific References　　(17)　　　　　\|　　　　　bs-10211R has been referenced in 17 publications. [IF=24.897]　Yuan, Xulei. et al. Systemic antibiotics increase microbiota pathogenicity and oral bone loss. INT J ORAL SCI. 2023 Jan;15(1):1-14　　IHC　;　　Mouse.　　 PubMed:36631439 [IF=15.304]　Jinbo Li. et al. Autophagy inhibition recovers deficient ICD-based cancer immunotherapy. BIOMATERIALS. 2022 Aug;287:121651　　IF　;　　Mouse.　　 PubMed:35777331 [IF=9.776]　Yingli Wang. et al. Paclitaxel derivative-based liposomal nanoplatform for potentiated chemo-immunotherapy. J Control Release. 2022 Jan;341:812　　IF　;　　Mouse.　　 PubMed:34953979 [IF=8.806]　Li TF et al. Dendritic cell-mediated delivery of doxorubicin-polyglycerol-nanodiamond composites elicits enhanced anti-cancer immune response in glioblastoma.Biomaterials. 2018 Oct;181:35-52.　　IHC-P　;　　Human.　　 PubMed:30071380 [IF=7.727]　Jinbo Li. et al. Low dose shikonin and anthracyclines coloaded liposomes induce robust immunogenetic cell death for synergistic chemo-immunotherapy. J Control Release. 2021 Jul;335:306　　IHC　;　　Mouse.　　 PubMed:34081995 [IF=7.425]　Guangmang Liu. et al. Dietary glutamate enhances intestinal immunity by modulating microbiota and Th17/Treg balance-related immune signaling in piglets after lipopolysaccharide challenge. FOOD RES INT. 2023 Apr;166:112597　　WB　;　　Pig.　　 PubMed:36914323 [IF=6.832]　Yun Yu. et al. Geniposide promotes splenic Treg differentiation to alleviate colonic inflammation and intestinal barrier injury in ulcerative colitis mice. BIOENGINEERED. 2022;13(6):14616-14631　　FCM　;　　Mouse.　　 PubMed:36694912 [IF=5.396]　Yan Zhang. et al. Polysaccharide from Ganoderma lucidum Alleviates Congnitive Impairment in a Mice Model of Chronic Cerebral Hypoperfusion by Regulating CD4+CD25+Foxp3+ regulatory T cells. Food Funct. 2022 Jan;:　　WB　;　　Mouse.　　 PubMed:35088782 [IF=5.345]　Yuan SJ et al. Doxorubicin-polyglycerol-nanodiamond conjugate is a cytostatic agent that evades chemoresistance and reverses cancer-induced immunosuppression in triple-negative breast cancer.J Nanobiotechnology. 2019 Oct 17;17(1):110.　　IHC-P　;　　Mouse.　　 PubMed:31623629 [IF=5.279]　Yan Zhang. et al. Ganoderic Acid A To Alleviate Neuroinflammation of Alzheimer’s Disease in Mice by Regulating the Imbalance of the Th17/Tregs Axis. J Agr Food Chem. 2021;69(47):14204–14214　　WB　;　　Mouse.　　 PubMed:34798773 [IF=4.2]　Shuyu Ge. et al. Wuweixiaoduyin regulates TAZ-mediated immunoregulatory properties of Treg/TH17 cells in chronic osteomyelitis. BIOTECHNOL GENET ENG. 2023 Jan 14　　WB　;　　Rat.　　 PubMed:36641597 [IF=3.943]　Quanyu Chen. et al. Hepatocyte growth factor mediates a novel form of hepatic stem/progenitor cell-induced tolerance in a rat xenogeneic liver rejection model. Int Immunopharmacol. 2021 Jan;90:107180　　IHC　;　　Hamster, Rat.　　 PubMed:33221167 [IF=3.513]　Shanshan Yang. et al. Linoleic acid stimulation results in TGF-β1 production and inhibition of PEDV infection in vitro. VIROLOGY. 2023 Apr;581:89　　FCM　;　　Pig.　　 PubMed:36934552 [IF=3.457]　Zheng X et al. Dendritic cells and Th17/Treg ratio play critical roles in pathogenic process of chronic obstructive pulmonary disease. (2018) Biomedicine & Pharmacotherapy.108,1141–1151.　　IHC　;　　Human.　　 PubMed:30372815 [IF=3.411]　Yan Zheng. et al. Downregulation of Rap1GAP Expression Activates the TGF-β/Smad3 Pathway to Inhibit the Expression of Sodium/Iodine Transporter in Papillary Thyroid Carcinoma Cells. Biomed Res Int. 2021;2021:6840642　　WB　;　　Human.　　 PubMed:34840979 [IF=2.65]　Liu Liping. et al. Sodium Butyrate Attenuated Diabetes-Induced Intestinal Inflammation by Modulating Gut Microbiota. EVID-BASED COMPL ALT. 2022;2022:4646245　　WB　;　　Mouse.　　 PubMed:36045662 [IF=2.251]　Xinhua Liet al. Upregulation of microRNA-219-5p relieves ulcerative colitis through balancing the differentiation of Treg/Th17 cells. Eur J Gastroenterol Hepatol. 2020 Jul;32(7):813-820.　　WB　;　　mouse.　　 PubMed:32175983
研究領(lǐng)域	轉(zhuǎn)錄調(diào)節(jié)因子
抗體來(lái)源	Rabbit
克隆類型	Polyclonal
交叉反應(yīng)	Human,Mouse,Rat (predicted: Dog,Pig,Cow,Horse,Rabbit,Sheep,GuineaPig)
產(chǎn)品應(yīng)用	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100, IF=1:100-500, Flow-Cyt=0.2ug/Test, ELISA=1:5000-10000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
理論分子量	47kDa
細(xì)胞定位	細(xì)胞核
性　　　　狀	Liquid
濃　　　　度	1mg/ml
免　疫　原	KLH conjugated synthetic peptide derived from human FoxP3:　331-431/431
亞　　　　型	IgG
純化方法	affinity purified by Protein A
緩　沖　液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng)	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
產(chǎn)品介紹	The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]. Function: Probable transcription factor. Plays a critical role in the control of immune response. Subunit: Interacts with IKZF3. Subcellular Location: Nucleus (Potential). Post-translational modifications: Acetylation on lysine residues stabilizes FOXP3 and promotes differentiation of T-cells into induced regulatory T-cells (iTregs) associated with suppressive functions. Deacetylated by SIRT1. DISEASE: Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) [MIM:304790]; also known as X-linked autoimmunity-immunodeficiency syndrome. IPEX is characterized by neonatal onset insulin-dependent diabetes mellitus, infections, secretory diarrhea, trombocytopenia, anemia and eczema. It is usually lethal in infancy. Similarity: Contains 1 C2H2-type zinc finger. Contains 1 fork-head DNA-binding domain. SWISS: Q9BZS1 Gene ID: 50943 Database links: Entrez Gene: 50943 Human Entrez Gene: 20371 Mouse Entrez Gene: 317382 Rat Omim: 300292 Human SwissProt: Q9BZS1 Human SwissProt: Q99JB6 Mouse SwissProt: D3ZKI1 Rat Unigene: 247700 Human Unigene: 182291 Mouse
產(chǎn)品圖片	Sample: Spleen (Rat) Lysate at 40 ug Primary: Anti-FoxP3 (bs-10211R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 47 kD Observed band size: 47 kD Protein: Raji(human) lysates at 40ug; Primary: rabbit Anti-FoxP3 (bs-10211R) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000; Predicted band size:47 kD Observed band size:47 kD Sample: Thymus (Mouse) Lysate at 40 ug Primary: Anti-FoxP3 (bs-10211R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 47 kD Observed band size: 47 kD Sample: Lane 1: PBMC (Mouse) Lysate at 40 ug Lane 2: Thymus (Mouse) Lysate at 40 ug Lane 3: Spleen (Mouse) Lysate at 40 ug Lane 4: Thymus (Rat) Lysate at 40 ug Primary: Anti-FoxP3 (bs-10211R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 43/45 kD Observed band size: 43/45 kD Sample: U937 Cell (Human) Lysate at 30 ug Primary: Anti-FoxP3 (Bs-10211R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 47 kD Observed band size: 48 kD Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-FoxP3 Polyclonal Antibody, Unconjugated(bs-10211R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: rat spleen; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-FoxP3 Polyclonal Antibody, Unconjugated(bs-10211R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Blank control: Mouse spleen. Primary Antibody (green line): Rabbit Anti-FoxP3/PE Conjugated antibody (bs-10211R-PE) Dilution: 0.2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG-PE . Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed. Blank control: MCF7. Primary Antibody (green line): Rabbit Anti-FoxP3 antibody (bs-10211R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. Blank control: MCF7. Primary Antibody (green line): Rabbit Anti-FoxP3 antibody (bs-10211R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. Blank control (Black line):Mouse spleen (Black). Primary Antibody (green line): Rabbit Anti-FoxP3 antibody (bs-10211R) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA goat serum to block non-specific protein-protein interactions for 15 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.