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產(chǎn)品編號(hào)	bsm-33190M
英文名稱	Mouse　Anti-Caspase 8　antibody
中文名稱	半胱氨酸蛋白酶8單克隆抗體
別　　　　名	Caspase-8 subunit p10; ALPS2B; Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 12 protein; Apoptosis related cysteine peptidase; Apoptotic cysteine protease; Apoptotic protease Mch 5; Apoptotic protease Mch-5; Apoptotic protease Mch5; CAP 4; CAP4; CASP 8; CASP-8; Caspase-8; Caspase8; CASP8; CASP8_HUMAN; Caspase8; Caspase 8 apoptosis related cysteine peptidase; Caspase8; CED 3; FADD homologous ICE/CED 3 like protease; FADD Homologous ICE/CED3 Like Protease; FADD Like ICE antibody FADD-homologous ICE/CED-3-like protease; FADD-like ICE; FLICE; FLJ17672; ICE like apoptotic protease 5; ICE-like apoptotic protease 5; MACH alpha 1/2/3 protein; MACH antibody MACH beta 1/2/3/4 protein antibody MCH 5; MCH5.
	Specific References　　(9)　　　　　\|　　　　　bsm-33190M has been referenced in 9 publications. [IF=6.291]　Peng Zheng. et al. Alleviative effect of melatonin on the decrease of uterine receptivity caused by blood ammonia through ROS/NF-κB pathway in dairy cow. Ecotox Environ Safe. 2022 Feb;231:113166　　WB　;　　Bovine.　　 PubMed:35030520 [IF=5.89]　Rentao Zhang. et al. Anti-Gastric Cancer Activity of the Cell-free Culture Supernatant of Serofluid Dish and Lactiplantibacillus plantarum YT013. FRONT BIOENG BIOTECH. 2022; 10: 898240　　WB　;　　Human.　　 PubMed:35677304 [IF=5.162]　Serag Eldin I. Elbehairi. et al. Chitosan nano-vehicles as biocompatible delivering tools for a new Ag(I)curcuminoid-Gboxin analog complex in cancer and inflammation therapy. Int J Biol Macromol. 2020 Dec;165:2750　　WB　;　　Human.　　 PubMed:10.1016/j.ijbiomac.2020.10.153 [IF=5.123]　Rentao Zhang. et al. Exopolysaccharide from Lactiplantibacillus plantarum YT013 and Its Apoptotic Activity on Gastric Cancer AGS Cells. Fermentation-Basel. 2023 Jun;9(6):539　　WB　;　　Human.　　 PubMed:10.3390/fermentation9060539 [IF=5.016]　Min-Lin He. et al. Screening and characterization of a novel efficient tumor cell-targeting peptide derived from insulin-like growth factor binding proteins. J DRUG TARGET. 2023 Mar 28　　WB　;　　Human.　　 PubMed:36974745 [IF=4.462]　Zhao W et al. Junduqing extractive promotes the apoptosis of nasopharyngeal carcinoma cells through down-regulating Mcl-1 and Bcl-xL and up-regulating Caspase-3, Caspase-8 and Caspase-9. Artif Cells Nanomed Biotechnol. 2019 Dec;47(1):3904-3912.　　WB　;　　Human.　　 PubMed:31566021 [IF=3.448]　Xu B et al. Excessive?mechanical?stress?induces?chondrocyte?apoptosis?through?TRPV4?in an?anteriorcruciate?ligament-transected?rat?osteoarthritis?model. Life Sci. 2019 Jul 1;228:158-166.　　WB　;　　Rat.　　 PubMed:31055086 [IF=3.417]　Lei Xiang. et al. Antitumor effects of curcumin on the proliferation, migration and apoptosis of human colorectal carcinoma HCT?116 cells. Oncol Rep. 2020 Nov;44(5):1997-2008　　WB　;　　Human.　　 PubMed:33000266 [IF=3.067]　Kang Z et al. Copper-induced apoptosis and autophagy through oxidative stress-mediated mitochondrial dysfunction in male germ cells. Toxicol In Vitro. 2019 Sep 3;61:104639.　　WB　;　　Mouse.　　 PubMed:31491480
研究領(lǐng)域	細(xì)胞生物　　神經(jīng)生物學(xué)　　細(xì)胞凋亡
抗體來(lái)源	Mouse
克隆類型	Monoclonal
克　隆　號(hào)	4G12
交叉反應(yīng)	Human,Mouse (predicted: Rat)
產(chǎn)品應(yīng)用	WB=1:2000-5000, IHC-P=1:400-1000, IHC-F=1:500-1000, IF=1:500-1000, ELISA=1:5000-10000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
理論分子量	43/57kDa
檢測(cè)分子量	43kDa
細(xì)胞定位	細(xì)胞漿
性　　　　狀	Liquid
濃　　　　度	1mg/ml
免　疫　原	Recombinant human Caspase 8 Protein
亞　　　　型	IgG
純化方法	affinity purified by Protein G
緩　沖　液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng)	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
產(chǎn)品介紹	Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase 8 and Caspase 9. Activated initiator caspases then process effector caspases, such as Caspase 3 and Caspase 7, which in turn cause cell collapse. Function: Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-\|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex. Subunit: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 18 kDa (p18) and a 10 kDa (p10) subunit. Interacts with FADD, CFLAR and PEA15. Isoform 9 interacts at the endoplasmic reticulum with a complex containing BCAP31, BAP29, BCL2 and/or BCL2L1. Interacts with TNFAIP8L2. Subcellular Location: Cytoplasm. Tissue Specificity: Isoform 1, isoform 5 and isoform 7 are expressed in a wide variety of tissues. Highest expression in peripheral blood leukocytes, spleen, thymus and liver. Barely detectable in brain, testis and skeletal muscle. DISEASE: Defects in CASP8 are the cause of caspase-8 deficiency (CASP8D) [MIM:607271]. CASP8D is a disorder resembling autoimmune lymphoproliferative syndrome (ALPS). It is characterized by lymphadenopathy, splenomegaly, and defective CD95-induced apoptosis of peripheral blood lymphocytes (PBLs). It leads to defects in activation of T-lymphocytes, B-lymphocytes, and natural killer cells leading to immunodeficiency characterized by recurrent sinopulmonary and herpes simplex virus infections and poor responses to immunization. Similarity: Belongs to the peptidase C14A family. Contains 2 DED (death effector) domains. SWISS: Q14790 Gene ID: 841 Database links: Entrez Gene: 841　Human Entrez Gene: 12370　Mouse Entrez Gene: 54474　Rat Entrez Gene: 64044　Rat Omim: 601763　Human SwissProt: Q14790　Human SwissProt: O89110　Mouse SwissProt: Q9JHX4　Rat Unigene: 599762　Human Unigene: 655983　Human Unigene: 336851　Mouse 半胱氨酸蛋白酶-8，在凋亡執(zhí)行階段起中心作用。
產(chǎn)品圖片	Sample: Hela(Human) Cell Lysate at 30 ug Jurkat(Human) Cell Lysate at 30 ug Molt-4(Human) Cell Lysate at 30 ug K562(Human) Cell Lysate at 30 ug Primary: Anti-Caspase 8 (bsm-33190M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41'43'55 kD Observed band size: 43 kD Sample: 293T-UV(Human) Cell Lysate at 30 ug U251-UV(Human) Cell Lysate at 30 ug Primary: Anti-Caspase 8 (bsm-33190M) at 1/4000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 41’43’55 kD Observed band size: 43 kD Sample: Stomach(Mouse) Lysate at 40 ug Spleen(Mouse) Lysate at 40 ug Lymph node(Mouse) Lysate at 40 ug Urinary bladder(Mouse) Lysate at 40 ug Primary: Anti-Caspase 8 (bsm-33190M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41'43'55 kD Observed band size: 55'53D Paraformaldehyde-fixed, paraffin embedded (Mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Caspase 8) Monoclonal Antibody, Unconjugated (bsm-33190M) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Caspase 8) Monoclonal Antibody, Unconjugated (cell supernatant of bsm-33190M 4G12) at overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Caspase 8) Monoclonal Antibody, Unconjugated (cell supernatant of bsm-33190M 4G12) at overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Caspase 8) Monoclonal Antibody, Unconjugated (cell supernatant of bsm-33190M 4G12) at overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat lymphoid); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Caspase 8) Monoclonal Antibody, Unconjugated (ascites of bsm-33190M 4G12) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat lymphoid); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Caspase 8) Monoclonal Antibody, Unconjugated (ascites of bsm-33190M 4G12) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat lymphoid); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Caspase 8) Monoclonal Antibody, Unconjugated (ascites of bsm-33190M 4G12) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat lymphoid); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Caspase 8) Monoclonal Antibody, Unconjugated (ascites of bsm-33190M 4G12) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.