| 產(chǎn)品編號 | bs-0735R |
| 英文名稱 | Rabbit Anti-FGF8 antibody |
| 中文名稱 | 成纖維細(xì)胞生長因子8抗體 |
| 別 名 | AIGF; Androgen induced growth factor; Androgen-induced growth factor; FGF 8; FGF-8; FGF8; Fibroblast growth factor 8 (androgen induced); Fibroblast growth factor 8 precursor; HBGF 8; HBGF-8; HBGF8; FGF8_HUMAN. |
 | Specific References (2) | bs-0735R has been referenced in 2 publications. [IF=1.77] Lu et al. Changes in expression and secretion patterns of fibroblast growth factor 8 and Sonic Hedgehog signaling pathway molecules during murine neural stem/progenitor cell differentiation in vitro. (2012) Neural.Regen.Res. 7:1688-94 WB, IF(ICC) ; Mouse, Rat. [IF=1.559] Chen et al. Sonic hedgehog protein regulates fibroblast growth factor 8 expression in metanephric explant culture from BALB/c mice: Possible mechanisms associated with renal morphogenesis. (2016) Mol.Med.Re. 14:2929-36 IF(IHC-P) ; Mouse. |
| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 免疫學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Chicken,Dog,Pig,Cow,Sheep) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 27kDa |
| 細(xì)胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human FGF8: 131-233/233 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | Keratinocyte growth factor precursor (KGF) (Fibroblast growth factor 8; FGF-8; HBGF-8). Stimulates growth of the cells in an autocrine manner. Mediates hormonal action on the growth of cancer cells. Cooperates with Wnt-1 in mouse mammary tumor virus-induced murine mammary tumorigenesis. [Subcellular location] Secreted. [Tissue specificity] Absent in normal mammary glands and detected only in adult testis and ovary and in midgestational embryos. [Induction] By androgens. Belongs to the heparin-binding growth factors family. Function: Stimulates growth of the cells in an autocrine manner. Mediates hormonal action on the growth of cancer cells. Subunit: Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Subcellular Location: Secreted. DISEASE: Defects in FGF8 are the cause of Kallmann syndrome type 6 (KAL6) [MIM:612702]. Kallmann syndrome is a disorder that associates hypogonadotropic hypogonadism and anosmia. Anosmia or hyposmia is related to the absence or hypoplasia of the olfactory bulbs and tracts. Hypogonadism is due to deficiency in gonadotropin-releasing hormone and probably results from a failure of embryonic migration of gonadotropin-releasing hormone-synthesizing neurons. In some patients other developmental anomalies can be present, which include renal agenesis, cleft lip and/or palate, selective tooth agenesis, and bimanual synkinesis. In some cases anosmia may be absent or inconspicuous. Similarity: Belongs to the heparin-binding growth factors family. SWISS: P37237 Gene ID: 2253 Database links: Entrez Gene: 2253 Human Entrez Gene: 14179 Mouse Omim: 600483 Human SwissProt: P55075 Human SwissProt: P37237 Mouse Unigene: 57710 Human Unigene: 4012 Mouse Unigene: 73565 Rat 有研究者認(rèn)為：纖維母細(xì)胞生長因子8 FGF8 對癌癥具有一定的抑制作用。 早期研究FGF8，認(rèn)為是自分泌生長因子，它在前列腺癌、乳癌和卵巢癌中常常表現(xiàn)過量表達(dá) (over-expressed)，并與前列腺癌的格里森分?jǐn)?shù) (Gleason score) 相關(guān)聯(lián)。經(jīng)過各種細(xì)胞和動物模型的實驗均支持FGF8在抑制腫瘤形成和腫瘤細(xì)胞成活方面的作用。 此外，還有學(xué)者認(rèn)為：FGF8 還在實驗中顯示出，他有改善因膠原蛋白引發(fā)的關(guān)節(jié)炎性的改變，如：風(fēng)濕性關(guān)節(jié)炎和類風(fēng)濕性關(guān)節(jié)炎及退行性骨性關(guān)節(jié)病。 |
| 產(chǎn)品圖片 |  Paraformaldehyde-fixed, paraffin embedded (rat ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FGF8) Polyclonal Antibody, Unconjugated (bs-0735R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.  Paraformaldehyde-fixed, paraffin embedded (rat ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FGF8) Polyclonal Antibody, Unconjugated (bs-0735R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.  Paraformaldehyde-fixed, paraffin embedded (rat ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FGF8) Polyclonal Antibody, Unconjugated (bs-0735R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.  Paraformaldehyde-fixed, paraffin embedded (human prostate); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FGF8) Polyclonal Antibody, Unconjugated (bs-0735R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.  Paraformaldehyde-fixed, paraffin embedded (human prostate); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FGF8) Polyclonal Antibody, Unconjugated (bs-0735R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.  Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FGF8) Polyclonal Antibody, Unconjugated (bs-0735R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.  Paraformaldehyde-fixed, paraffin embedded (mouse ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FGF8) Polyclonal Antibody, Unconjugated (bs-0735R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.  Paraformaldehyde-fixed, paraffin embedded (mouse ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FGF8) Polyclonal Antibody, Unconjugated (bs-0735R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.  Paraformaldehyde-fixed, paraffin embedded (rat prostate); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FGF8) Polyclonal Antibody, Unconjugated (bs-0735R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.  Paraformaldehyde-fixed, paraffin embedded (Rat ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FGF8) Polyclonal Antibody, Unconjugated (bs-0735R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining. |
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