| 產(chǎn)品編號 | bsm-52867R |
| 英文名稱 | Rabbit Anti-MYH6 antibody |
| 中文名稱 | 心肌肌球蛋白重鏈重組兔單抗 |
| 別 名 | MYH6_HUMAN; Myosin-6; MYHCA; Myosin heavy chain 6; Myosin heavy chain, cardiac muscle alpha isoform (MyHC-alpha); ASD3; MYHC; SSS3; CMH14; MYHCA; CMD1EE; alpha-MHC; Myosin heavy chain; |
 | Specific References (1) | bsm-52867R has been referenced in 1 publications. [IF=8.713] Zhao-Bo Luo. et al. Fecal transplant from myostatin deletion pigs positively impacts the gut-muscle axis. ELIFE. 2023; 12: e81858 WB ; Mouse. |
| 研究領(lǐng)域 | 心血管 免疫學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 8C1 |
| 交叉反應(yīng) | (predicted: Human,Mouse,Rat) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000, IHC-P=1:100-500, IF=1:100 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 224kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human MYH6 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | Cardiac muscle myosin is a hexamer consisting of two heavy chain subunits, two light chain subunits, and two regulatory subunits. This gene encodes the alpha heavy chain subunit of cardiac myosin. The gene is located approximately 4kb downstream of the gene encoding the beta heavy chain subunit of cardiac myosin. Mutations in this gene cause familial hypertrophic cardiomyopathy and atrial septal defect 3. [provided by RefSeq, Feb 2017] Subcellular Location: Cytoplasm, myofibril. Note=Thick filaments of the myofibrils. SWISS: P13533 Gene ID: 4624 Database links: Entrez Gene: 4624 Human Entrez Gene: 17888 Mouse SwissProt: P13533 Human SwissProt: Q02566 Mouse |
| 產(chǎn)品圖片 |  Western blot analysis of Myosin heavy chain on human skeletal muscle tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.  Paraformaldehyde-fixed, paraffin embedded (mouse heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MYH6) Monoclonal Antibody, Unconjugated (bsm-52867R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023)instructionsand DAB staining.  Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MYH6) Monoclonal Antibody, Unconjugated (bsm-52867R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023)instructionsand DAB staining.  Paraformaldehyde-fixed, paraffin embedded (human skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MYH6) Monoclonal Antibody, Unconjugated (bsm-52867R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023)instructionsand DAB staining.  Paraformaldehyde-fixed, paraffin embedded (mouse smooth muscle tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MYH6) Monoclonal Antibody, Unconjugated (bsm-52867R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023)instructionsand DAB staining.  Paraformaldehyde-fixed, paraffin embedded (rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MYH6) Monoclonal Antibody, Unconjugated (bsm-52867R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023)instructionsand DAB staining.  Paraformaldehyde-fixed, paraffin embedded (rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MYH6) Monoclonal Antibody, Unconjugated (bsm-52867R) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-AF488) for 90 minutes, and DAPI for nuclei staining.  Paraformaldehyde-fixed, paraffin embedded (mouse heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MYH6) Monoclonal Antibody, Unconjugated (bsm-52867R) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-AF488) for 90 minutes, and DAPI for nuclei staining.  Paraformaldehyde-fixed, paraffin embedded (human heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MYH6) Monoclonal Antibody, Unconjugated (bsm-52867R) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-AF488) for 90 minutes, and DAPI for nuclei staining.  Paraformaldehyde-fixed, paraffin embedded (Human striated muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MYH6) Monoclonal Antibody, Unconjugated (bsm-52867R) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-AF488) for 90 minutes, and DAPI for nuclei staining. |
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