| 產品編號 | bsm-60751R |
| 英文名稱 | Rabbit Anti-Heme Oxygenase 1 antibody |
| 中文名稱 | 血紅素氧合酶 1抗體 |
| 別 名 | Heme oxygenase (decycling) 1; Heme oxygenase 1; Hemox; HMOX 1; Hmox; HMOX1; HO 1; HO-1; HO; HO1; Hsp32; bK286B10; D8Wsu38e; HMOX1_HUMAN. |
| 研究領域 | 腫瘤 心血管 免疫學 神經生物學 信號轉導 表觀遺傳學 Alzheimer's |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 交叉反應 | Rat,Mouse,Human |
| 產品應用 | WB=1:2000-20000, IHC-P=1:100-500, ICC=1:50, IF=1:50 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 32kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Heme Oxygenase 1 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | PBS, Glycerol, BSA |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 | The hemeoxygenase-1 calls that the hemoglobin oxidizes to synthesize the enzyme again-1( hemeoxygenase-1, HO-1) is the catalyst enzyme that a kind of hemoglobin declines the solution, under the NADPH and the cell dye P-450 revivification enzymes and the member oxygen functions, the catalyst HO-1 hemoglobin declines the solution as the courage green vegetable, CO and irons, the former revivification has the very strong anti- to oxidize the ability after become the red vegetable of courage , the latter is a kind of important letter to make the member. SWISS: P09601 Gene ID: 3162 |
| 產品圖片 |  Sample: Lane 1: Mouse Liver tissue lysates Lane 2: Mouse Placenta tissue lysates Lane 3: Mouse NIH/3T3 cell lysates Lane 4: Rat Spleen tissue lysates Lane 5: Rat Liver tissue lysates Lane 6: Human HeLa cell lysates Lane 7: Human 293T cell lysates Lane 8: Human HepG2 cell lysates Primary: Anti-Heme Oxygenase 1 (bsm-60751R) at 1/10000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 32 kDa Observed band size: 30 kDa  Paraformaldehyde-fixed, paraffin embedded (mouse thymus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Heme Oxygenase 1) Monoclonal Antibody, Unconjugated (bsm-60751R) at 1:1000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.  Paraformaldehyde-fixed, paraffin embedded (human spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Heme Oxygenase 1) Monoclonal Antibody, Unconjugated (bsm-60751R) at 1:1000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.  Paraformaldehyde-fixed, paraffin embedded (human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Heme Oxygenase 1) Monoclonal Antibody, Unconjugated (bsm-60751R) at 1:1000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.  Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Heme Oxygenase 1) Monoclonal Antibody, Unconjugated (bsm-60751R) at 1:1000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.  Paraformaldehyde-fixed, paraffin embedded (rat thymus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Heme Oxygenase 1) Monoclonal Antibody, Unconjugated (bsm-60751R) at 1:1000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.  Paraformaldehyde-fixed, paraffin embedded (rat spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Heme Oxygenase 1) Monoclonal Antibody, Unconjugated (bsm-60751R) at 1:1000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.  Tissue: mouse liver Section type: Formalin-fixed & Paraffinembedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:2000 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab:SP Kit(Rabbit) (sp-0023) Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60751R  Tissue: rat liver Section type: Formalin-fixed & Paraffinembedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:2000 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab:SP Kit(Rabbit) (sp-0023) Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60751R  Cell line: MCF7 Fixative: 100% Ice-cold methanol Permeabilization: 0.1% TritonX-100 Primary ab dilution: 1:50 Primary incubation condition: 4°C overnight Secondary ab: Goat Anti-Rabbit IgG Nuclear counter stain: DAPI (Blue) Comment: Color green is the positive signal for bsm-60751R  Cell line: HeLa Fixative: 100% Ice-cold methanol Permeabilization: 0.1% TritonX-100 Primary ab dilution: 1:50 Primary incubation condition: 4°C overnight Secondary ab: Goat Anti-Rabbit IgG Nuclear counter stain: DAPI (Blue) Comment: Color green is the positive signal for bsm-60751R |
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