| 產(chǎn)品編號(hào) | bs-0598R |
| 英文名稱 | Rabbit Anti-DVL1 antibody |
| 中文名稱 | 蓬亂蛋白1抗體 |
| 別 名 | Dishevelled 1; Dishevelled; Dishevelled dsh homolog 1; Dishevelled1; DSH homolog 1; Dvl; MGC54245; Segment polarity protein dishevelled homolog DVL 1; Segment polarity protein dishevelled homolog DVL1; DVL1_HUMAN. |
 | Specific References (2) | bs-0598R has been referenced in 2 publications. [IF=1.851] Wang T et al. The peptide compound urantide regulates collagen metabolism in atherosclerotic rat hearts and inhibits the JAK2/STAT3 pathway. Mol Med Rep. 2020 Mar;21(3):1097-1106. IHF-P&WB ; Rat. [IF=0] Hua, Jun-yi, et al. Hua, Jun-yi, et al. "Wnt4/β-catenin signaling pathway modulates balloon-injured carotid artery restenosis via disheveled-1." Int J Clin Exp Pathol 7.12 (2014): 8421-8431. IHC-P ; Rat. |
| 研究領(lǐng)域 | 免疫學(xué) 神經(jīng)生物學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 76kDa |
| 細(xì)胞定位 | 細(xì)胞漿 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human DVL1: 21-100/695 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | DVL1 may play a role in the signal transduction pathway mediated by multiple Wnt genes. [Subunit] Interacts with BRD7 and INVS. Interacts through its PDZ domain with the C-terminal regions of VANGL1, VANGL2 and CCDC88C/DAPLE. Interacts (via PDZ domain) with NXN (By similarity). Interacts with CXXC4. [Subcellular Location] Cytoplasm (Potential). Belongs to the DSH family. Function: Participates in Wnt signaling by binding to the cytoplasmic C-terminus of frizzled family members and transducing the Wnt signal to down-stream effectors. Plays a role both in canonical and non-canonical Wnt signaling. Plays a role in the signal transduction pathways mediated by multiple Wnt genes. Required for LEF1 activation upon WNT1 and WNT3A signaling. DVL1 and PAK1 form a ternary complex with MUSK which is important for MUSK-dependent regulation of AChR clustering during the formation of the neuromuscular junction (NMJ). Subunit: Interacts with CXXC4. Interacts (via PDZ domain) with NXN (By similarity). Interacts with BRD7 and INVS. Interacts through its PDZ domain with the C-terminal regions of VANGL1, VANGL2 and CCDC88C/DAPLE. Interacts with ARRB1; the interaction is enhanced by phosphorylation of DVL1. Interacts with CYLD (By similarity). Interacts (via PDZ domain) with RYK. Self-associates (via DIX domain) and forms higher homooligomers. Interacts (via PDZ domain) with DACT1 and FZD7, where DACT1 and FZD7 compete for the same binding site (By similarity). Interacts (via DEP domain) with MUSK; the interaction is direct and mediates the formation a DVL1, MUSK and PAK1 ternary complex involved in AChR clustering (By similarity). Subcellular Location: Cell membrane. Cytoplasm, cytosol. Cytoplasmic vesicle. Localizes at the cell membrane upon interaction with frizzled family members. Post-translational modifications: Ubiquitinated; undergoes both 'Lys-48'-linked ubiquitination, leading to its subsequent degradation by the ubiquitin-proteasome pathway, and 'Lys-63'-linked ubiquitination. The interaction with INVS is required for ubiquitination. Deubiquitinated by CYLD, which acts on 'Lys-63'-linked ubiquitin chains. Similarity: Belongs to the DSH family. Contains 1 DEP domain. Contains 1 DIX domain. Contains 1 PDZ (DHR) domain. SWISS: O14640 Gene ID: 1855 Database links: Entrez Gene: 1855 Human Entrez Gene: 13542 Mouse Omim: 601365 Human SwissProt: Q5IS48 Chimpanzee SwissProt: O14640 Human SwissProt: P51141 Mouse Unigene: 741165 Human Unigene: 3400 Mouse Unigene: 144610 Rat dishevelled-1蓬亂蛋白-1主要用于神經(jīng)退行性改變-AD病方面的研究. |
| 產(chǎn)品圖片 |  Protein: Brain(Rat) lysate at 30ug; Lung(Rat) lysate at 30ug; Primary: Anti-DVL1/Dishevelled (bs-0598R) at 1:200 dilution; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution; Predicted band size : 76kD Observed band size : 76kD  Sample: Lane 1: Human HepG2 cell lysates Lane 2: Human Hela cell lysates Primary: Anti-DVL1 (bs-0598R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 76 kDa Observed band size: 75 kDa  Sample: Testis (Mouse) Lysate at 40 ug NIH/3T3 (Mouse) Cell Lysate at 30 ug Primary: Anti-DVL1 (bs-0598R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 76 kD Observed band size: 77 kD  Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DVL1) Polyclonal Antibody, Unconjugated (bs-0598R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.  Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DVL1) Polyclonal Antibody, Unconjugated (bs-0598R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.  Images provided the Independent Validation Program (badge number 029657)Formalin-fixed and paraffin embedded human kidney labeled with Rabbit Anti-DVL1 Polyclonal Antibody (bs-0598R) at 1:250 overnight at room temperature followed by conjugation to secondary antibody.  Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-DVL1/Dishevelled Polyclonal Antibody, Unconjugated(bs-0598R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
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